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  Prostaglandin E-1 inhibits endocytosis in the beta-cell endocytosis.

Zhao, Y., Fang, Q., Straub, S. G., Lindau, M., & Sharp, G. W. G. (2016). Prostaglandin E-1 inhibits endocytosis in the beta-cell endocytosis. Journal of Endocrinology, 229(3), 287-294. doi:10.1530/JOE-15-0435.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002B-1909-2 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002B-190F-5
Genre: Journal Article

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 Creators:
Zhao, Y.1, Author              
Fang, Q.1, Author              
Straub, S. G., Author
Lindau, M.1, Author              
Sharp, G. W. G., Author
Affiliations:
1Research Group of Nanoscale Cell Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_1832294              

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Free keywords: prostaglandin E-1; endocytosis; heterotrimeric G-protein Gz; beta-cells
 Abstract: Prostaglandins inhibit insulin secretion in a manner similar to that of norepinephrine (NE) and somatostatin. As NE inhibits endocytosis as well as exocytosis, we have now examined the modulation of endocytosis by prostaglandin E-1 (PGE(1)). Endocytosis following exocytosis was recorded by whole-cell patch clamp capacitance measurements in INS-832/13 cells. Prolonged depolarizing pulses producing a high level of Ca2+ influx were used to stimulate maximal exocytosis and to deplete the readily releasable pool (RRP) of granules. This high Ca2+ influx eliminates the inhibitory effect of PGE(1) on exocytosis and allows specific characterization of the inhibitory effect of PGE(1) on the subsequent compensatory endocytosis. After stimulating exocytosis, endocytosis was apparent under control conditions but was inhibited by PGE(1) in a Pertussis toxin-sensitive (PTX)-insensitive manner. Dialyzing a synthetic peptide mimicking the C-terminus of the alpha-subunit of the heterotrimeric G-protein G(z) into the cells blocked the inhibition of endocytosis by PGE(1), whereas a control-randomized peptide was without effect. These results demonstrate that PGE(1) inhibits endocytosis and G(z) mediates the inhibition.

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Language(s): eng - English
 Dates: 2016-04-112016-06
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1530/JOE-15-0435
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Title: Journal of Endocrinology
Source Genre: Journal
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Pages: - Volume / Issue: 229 (3) Sequence Number: - Start / End Page: 287 - 294 Identifier: -