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  AP-1/sigma 1A and AP-1/sigma 1B adaptor-proteins differentially regulate neuronal early endosome maturation via the Rab5/Vps34-pathway.

Candiello, E., Kratzke, M., Wenzel, D., Cassel, D., & Schu, P. (2016). AP-1/sigma 1A and AP-1/sigma 1B adaptor-proteins differentially regulate neuronal early endosome maturation via the Rab5/Vps34-pathway. Scientific Reports, 6: 29950. doi:10.1038/srep29950.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002B-1941-2 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-9EB3-B
Genre: Journal Article

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Candiello, E., Author
Kratzke, M., Author
Wenzel, D.1, Author              
Cassel, D., Author
Schu, P., Author
Affiliations:
1Facility for Electron Microscopy, MPI for biophysical chemistry, Max Planck Society, ou_578615              

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 Abstract: The sigma 1 subunit of the AP-1 clathrin-coated-vesicle adaptor-protein complex is expressed as three isoforms. Tissues express sigma 1A and one of the sigma 1B and sigma 1C isoforms. Brain is the tissue with the highest sigma 1A and sigma 1B expression. sigma 1B-deficiency leads to severe mental retardation, accumulation of early endosomes in synapses and fewer synaptic vesicles, whose recycling is slowed down. AP-1/sigma 1A and AP-1/sigma 1B regulate maturation of these early endosomes into multivesicular body late endosomes, thereby controlling synaptic vesicle protein transport into a degradative pathway. sigma 1A binds ArfGAP1, and with higher affinity brain-specific ArfGAP1, which bind Rabex-5. AP-1/sigma 1A-ArfGAP1-Rabex-5 complex formation leads to more endosomal Rabex-5 and enhanced, Rab5(GTP)-stimulated Vps34 PI3-kinase activity, which is essential for multivesicular body endosome formation. Formation of AP-1/sigma 1A-ArfGAP1-Rabex-5 complexes is prevented by sigma 1B binding of Rabex-5 and the amount of endosomal Rabex-5 is reduced. AP-1 complexes differentially regulate endosome maturation and coordinate protein recycling and degradation, revealing a novel molecular mechanism by which they regulate protein transport besides their established function in clathrin-coated-vesicle formation.

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Language(s): eng - English
 Dates: 2016-07-14
 Publication Status: Published online
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 Rev. Method: Peer
 Identifiers: DOI: 10.1038/srep29950
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Title: Scientific Reports
Source Genre: Journal
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Pages: 12 Volume / Issue: 6 Sequence Number: 29950 Start / End Page: - Identifier: -