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  Tissue Engineering of Cartilage on Ground-Based Facilities

Aleshcheva, G., Bauer, J., Hemmersbach, R., Egli, M., Wehland, M., & Grimm, D. (2016). Tissue Engineering of Cartilage on Ground-Based Facilities. Microgravity Science and Technology, 28(3), 237-245. doi:10.1007/s12217-015-9479-0.

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 Creators:
Aleshcheva, Ganna1, Author
Bauer, Johann2, Author              
Hemmersbach, Ruth1, Author
Egli, Marcel1, Author
Wehland, Markus1, Author
Grimm, Daniela1, Author
Affiliations:
1external, ou_persistent22              
2Scientific Service Groups, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565170              

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Free keywords: THYROID-CANCER CELLS; RANDOM POSITIONING MACHINE; HUMAN ENDOTHELIAL-CELLS; SIMULATED MICROGRAVITY; ARTICULAR-CARTILAGE; SPHEROID FORMATION; CULTURE-SYSTEM; STEM-CELLS; CYTOSKELETON; CHONDROCYTESMicrogravity; Random Positioning Machine; Clinostat; Rotating Wall Vessel; Chondrocytes; Cartilage; Cytoskeleton; Tissue engineering;
 Abstract: Investigations under simulated microgravity offer the opportunity for a better understanding of the influence of altered gravity on cells and the scaffold-free three-dimensional (3D) tissue formation. To investigate the short-term influence, human chondrocytes were cultivated for 2 h, 4 h, 16 h, and 24 h on a 2D Fast-Rotating Clinostat (FRC) in DMEM/F-12 medium supplemented with 10 % FCS. We detected holes in the vimentin network, perinuclear accumulations of vimentin after 2 h, and changes in the chondrocytes shape visualised by F-actin staining after 4 h of FRC-exposure. Scaffold-free cultivation of chondrocytes for 7 d on the Random Positioning Machine (RPM), the FRC and the Rotating Wall Vessel (RWV) resulted in spheroid formation, a phenomenon already known from spaceflight experiments with chondrocytes (MIR Space Station) and thyroid cancer cells (SimBox/Shenzhou-8 space mission). The experiments enabled by the ESA-CORA-GBF programme gave us an optimal opportunity to study gravity-related cellular processes, validate ground-based facilities for our chosen cell system, and prepare long-term experiments under real microgravity conditions in space.

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Language(s): eng - English
 Dates: 2016
 Publication Status: Published in print
 Pages: 9
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 000379321900006
DOI: 10.1007/s12217-015-9479-0
 Degree: -

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Title: Microgravity Science and Technology
  Abbreviation : Microgravity Sci. Technol.
Source Genre: Journal
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Publ. Info: Springer International Publishing AG
Pages: - Volume / Issue: 28 (3) Sequence Number: - Start / End Page: 237 - 245 Identifier: ISSN: 0938-0108
CoNE: https://pure.mpg.de/cone/journals/resource/0938-0108