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  The Impact of Hypergravity and Vibration on Gene and Protein Expression of Thyroid Cells

Wehland, M., Warnke, E., Frett, T., Hemmersbach, R., Hauslage, J., Ma, X., et al. (2016). The Impact of Hypergravity and Vibration on Gene and Protein Expression of Thyroid Cells. Microgravity Science and Technology, 28(3), 261-274. doi:10.1007/s12217-015-9474-5.

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 Creators:
Wehland, Markus1, Author
Warnke, Elisabeth1, Author
Frett, Timo1, Author
Hemmersbach, Ruth1, Author
Hauslage, Jens1, Author
Ma, Xiao1, Author
Aleshcheva, Ganna1, Author
Pietsch, Jessica1, Author
Bauer, Johann2, Author              
Grimm, Daniela1, Author
Affiliations:
1external, ou_persistent22              
2Scientific Service Groups, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565170              

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Free keywords: PARABOLIC FLIGHT MANEUVERS; ENDOTHELIAL GROWTH-FACTOR; ALTERED GRAVITY CONDITIONS; SIMULATED MICROGRAVITY; SPHEROID FORMATION; CANCER CELLS; HUMAN CHONDROCYTES; INTERMEDIATE-FILAMENTS; 3-DIMENSIONAL GROWTH; INCREASES APOPTOSISMicrogravity; Thyroid cells; Vibration; Hypergravity; Cytoskeleton; Integrins; Apoptosis; Signaling; Cell adhesion;
 Abstract: Experiments in space either on orbital missions on-board the ISS, or in suborbital missions using sounding rockets, like TEXUS as well as parabolic flight campaigns are still the gold standard to achieve real microgravity conditions in the field of gravitational biology and medicine. However, during launch, and in flight, hypergravity and vibrations occur which might interfere with the effects of microgravity. It is therefore important to know these effects and discriminate them from the microgravity effects. This can be achieved by ground-based facilities like centrifuges or vibration platforms. Recently, we have conducted several experiments with different thyroid cancer cell lines. This study, as part of the ESA-CORA-GBF 2010-203 project, focused on the influence of vibration and hypergravity on benign human thyroid follicular epithelial cells (Nthy-ori 3-1 cell line). Gene and in part protein expression regulation under both conditions were analyzed for VCAN, ITGA10, ITGB1, OPN, ADAM19, ANXA1, TNFA, ABL2, ACTB, PFN2, TLN1, EZR, RDX, MSN, CTGF, PRKCA, and PRKAA1 using quantitative real-time PCR and Western Blot. We found that hypergravity and vibration affected genes and proteins involved in the extracellular matrix, the cytoskeleton, apoptosis, cell growth and signaling. Vibration always led to a down-regulation, whereas hypergravity resulted in a more heterogeneous expression pattern. Overall we conclude that both conditions can influence gene regulation and production of various genes and proteins. As a consequence, it is important to perform control experiments on hypergravity and vibration facilities in parallel to flight experiments.

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Language(s): eng - English
 Dates: 2016
 Publication Status: Published in print
 Pages: 14
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 000379321900008
DOI: 10.1007/s12217-015-9474-5
 Degree: -

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Title: Microgravity Science and Technology
  Abbreviation : Microgravity Sci. Technol.
Source Genre: Journal
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Publ. Info: Springer International Publishing AG
Pages: - Volume / Issue: 28 (3) Sequence Number: - Start / End Page: 261 - 274 Identifier: ISSN: 0938-0108
CoNE: https://pure.mpg.de/cone/journals/resource/0938-0108