English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Fixed target combined with spectral mapping: approaching 100% hit rates for serial crystallography

Oghbaey, S., Sarracini, A., Ginn, H. M., Pare-Labrosse, O., Kuo, A., Marx, A., et al. (2016). Fixed target combined with spectral mapping: approaching 100% hit rates for serial crystallography. Acta Crystallographica Section D: Structural Biology, 72(8), 944-955. doi:10.1107/S2059798316010834.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002B-1D20-E Version Permalink: http://hdl.handle.net/21.11116/0000-0001-E035-6
Genre: Journal Article

Files

show Files
hide Files
:
gm5046.pdf (Publisher version), 2MB
 
File Permalink:
-
Name:
gm5046.pdf
Description:
-
Visibility:
Private
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Locator:
http://dx.doi.org/10.1107/S2059798316010834 (Publisher version)
Description:
-

Creators

show
hide
 Creators:
Oghbaey, Saeed1, Author
Sarracini, Antoine1, Author
Ginn, Helen M.2, 3, Author
Pare-Labrosse, Olivier1, Author
Kuo, Anling4, Author
Marx, Alexander5, Author              
Epp, Sascha W.5, Author              
Sherrell, Darren A.3, Author
Eger, Bryan T.4, Author
Zhong, Yin Peng5, Author              
Loch, Rolf5, Author              
Mariani, Valerio6, Author
Alonso-Mori, Roberto7, Author
Nelson, Silke7, Author
Lemke, Henrik T.7, Author
Owen, Robin L.3, Author
Pearson, Arwen R.8, Author
Stuart, David I.2, 3, Author
Ernst, Oliver P.4, 9, Author
Müller-Werkmeister, Henrike1, 4, 5, Author              
Miller, R. J. Dwayne1, 5, 9, Author               more..
Affiliations:
1Department of Physics and Chemistry, University of Toronto, 60 St George Street, Toronto, ON M5S 1A7, Canada, ou_persistent22              
2Division of Structural Biology, The Wellcome Trust Centre for Human Genetics, University of Oxford, Roosevelt Drive, Oxford OX3 7BN, England, ou_persistent22              
3Diamond Light Source, Harwell Science and Innovation Campus, Didcot OX11 ODE, England, ou_persistent22              
4Department of Biochemistry, University of Toronto, 1 King's College Circle, Toronto, Ontario, Canada, ou_persistent22              
5Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society, ou_1938288              
6Center for Free-Electron Laser Science, DESY, 22607 Hamburg, Germany, ou_persistent22              
7SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, CA 94025, USA, ou_persistent22              
8Hamburg Centre for Ultrafast Imaging, University of Hamburg, Hamburg, Germany, ou_persistent22              
9Department of Molecular Genetics, University of Toronto, 1 King's College Circle, Toronto, ON M5S 1A8, Canada, ou_persistent22              

Content

show
hide
Free keywords: femtosecond time-resolved crystallography; high-throughput serial crystallography; fixed-target sample delivery; room-temperature crystallography
 Abstract: The advent of ultrafast highly brilliant coherent X-ray free-electron laser sources has driven the development of novel structure-determination approaches for proteins, and promises visualization of protein dynamics on sub-picosecond timescales with full atomic resolution. Significant efforts are being applied to the development of sample-delivery systems that allow these unique sources to be most efficiently exploited for high-throughput serial femtosecond crystallography. Here, the next iteration of a fixed-target crystallography chip designed for rapid and reliable delivery of up to 11 259 protein crystals with high spatial precision is presented. An experimental scheme for predetermining the positions of crystals in the chip by means of in situ spectroscopy using a fiducial system for rapid, precise alignment and registration of the crystal positions is presented. This delivers unprecedented performance in serial crystallography experiments at room temperature under atmospheric pressure, giving a raw hit rate approaching 100% with an effective indexing rate of approximately 50%, increasing the efficiency of beam usage and allowing the method to be applied to systems where the number of crystals is limited.

Details

show
hide
Language(s): eng - English
 Dates: 2016-02-112016-07-042016-07-27
 Publication Status: Published online
 Pages: 12
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1107/S2059798316010834
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Acta Crystallographica Section D: Structural Biology
  Abbreviation : Acta Cryst. D
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Chester, England : International Union of Crystallography
Pages: - Volume / Issue: 72 (8) Sequence Number: - Start / End Page: 944 - 955 Identifier: ISSN: 2059-7983
CoNE: /journals/resource/2059-7983