English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Temperature dependence of water diffusion pools in brain white matter

Dhital, B., Labadie, C., Stallmach, F., Möller, H. E., & Turner, R. (2016). Temperature dependence of water diffusion pools in brain white matter. NeuroImage, 127, 135-143. doi:10.1016/j.neuroimage.2015.11.064.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002B-1FDF-3 Version Permalink: http://hdl.handle.net/21.11116/0000-0003-1DA8-0
Genre: Journal Article

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Dhital, Bibek1, Author              
Labadie, Christian2, 3, Author              
Stallmach, Frank3, Author
Möller, Harald E.2, Author              
Turner, Robert1, Author              
Affiliations:
1Department Neurophysics, MPI for Human Cognitive and Brain Sciences, Max Planck Society, ou_634550              
2Methods and Development Unit Nuclear Magnetic Resonance, MPI for Human Cognitive and Brain Sciences, Max Planck Society, ou_634558              
3Institute for Experimental Physics I, Faculty of Physics and Earth Sciences, University of Leipzig, Germany, ou_persistent22              

Content

show
hide
Free keywords: White matter; Diffusion; Activation energy; Myelin; Microtubules
 Abstract: Water diffusion in brain tissue can now be easily investigated using magnetic resonance (MR) techniques, providing unique insights into cellular level microstructure such as axonal orientation. The diffusive motion in white matter is known to be non-Gaussian, with increasing evidence for more than one water-containing tissue compartment. In this study, freshly excised porcine brain white matter was measured using a 125-MHz MR spectrometer (3 T) equipped with gradient coils providing magnetic field gradients of up to 35,000 mT/m. The sample temperature was varied between − 14 and + 19 °C. The hypothesis tested was that white matter contains two slowly exchanging pools of water molecules with different diffusion properties. A Stejskal–Tanner diffusion sequence with very short gradient pulses and b-factors up to 18.8 ms/μm2 was used. The dependence on b-factor of the attenuation due to diffusion was robustly fitted by a biexponential function, with comparable volume fractions for each component. The diffusion coefficient of each component follows Arrhenius behavior, with significantly different activation energies. The measured volume fractions are consistent with the existence of three water-containing compartments, the first comprising relatively free cytoplasmic and extracellular water molecules, the second of water molecules in glial processes, and the third comprising water molecules closely associated with membranes, as for example, in the myelin sheaths and elsewhere. The activation energy of the slow diffusion pool suggests proton hopping at the surface of membranes by a Grotthuss mechanism, mediated by hydrating water molecules.

Details

show
hide
Language(s): eng - English
 Dates: 2015-04-172015-11-252015-12-042016-02-15
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1016/j.neuroimage.2015.11.064
PMID: 26658929
Other: Epub 2015
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: NeuroImage
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Orlando, FL : Academic Press
Pages: - Volume / Issue: 127 Sequence Number: - Start / End Page: 135 - 143 Identifier: ISSN: 1053-8119
CoNE: /journals/resource/954922650166