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  Atom-scale depth localization of biologically important chemical elements in molecular layers

Schneck, E., Scoppola, E., Drnec, J., Mocuta, C., Felici, R., Novikov, D., et al. (2016). Atom-scale depth localization of biologically important chemical elements in molecular layers. Proceedings of the National Academy of Sciences of the United States of America, 113(34), 9521-9526. doi:10.1073/pnas.1603898113.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002B-297A-1 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002E-0B8F-D
Genre: Journal Article

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 Creators:
Schneck, Emanuel1, Author              
Scoppola, Ernesto, Author
Drnec, Jakub, Author
Mocuta, Cristian, Author
Felici, Roberto, Author
Novikov, Dmitri, Author
Fragneto, Giovanna, Author
Daillant, Jean, Author
Affiliations:
1Emanuel Schneck, Biomaterialien, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_2074300              

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Free keywords: Open Access
 Abstract: In nature, biomolecules are often organized as functional thin layers in interfacial architectures, the most prominent examples being biological membranes. Biomolecular layers play also important roles in context with biotechnological surfaces, for instance, when they are the result of adsorption processes. For the understanding of many biological or biotechnologically relevant phenomena, detailed structural insight into the involved biomolecular layers is required. Here, we use standing-wave X-ray fluorescence (SWXF) to localize chemical elements in solid-supported lipid and protein layers with near-Ångstrom precision. The technique complements traditional specular reflectometry experiments that merely yield the layers’ global density profiles. While earlier work mostly focused on relatively heavy elements, typically metal ions, we show that it is also possible to determine the position of the comparatively light elements S and P, which are found in the most abundant classes of biomolecules and are therefore particularly important. With that, we overcome the need of artificial heavy atom labels, the main obstacle to a broader application of high-resolution SWXF in the fields of biology and soft matter. This work may thus constitute the basis for the label-free, element-specific structural investigation of complex biomolecular layers and biological surfaces.

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 Dates: 2016-08-082016-08-23
 Publication Status: Published in print
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Title: Proceedings of the National Academy of Sciences of the United States of America
  Abbreviation : PNAS
Source Genre: Journal
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Publ. Info: Washington, D.C. : National Academy of Sciences
Pages: - Volume / Issue: 113 (34) Sequence Number: - Start / End Page: 9521 - 9526 Identifier: ISSN: 0027-8424