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  Local pH Gradient Initiated by Light on TiO2 for Light-Triggered Modulation of Polyhistidine-Tagged Proteins

Andreeva, D. V., Melnyk, I., Baidukova, O., & Skorb, E. V. (2016). Local pH Gradient Initiated by Light on TiO2 for Light-Triggered Modulation of Polyhistidine-Tagged Proteins. ChemElectroChem, 3(9), 1306-1310. doi:10.1002/celc.201600268.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002B-29FD-E Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002B-5FDF-8
Genre: Journal Article

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 Creators:
Andreeva, Daria V., Author
Melnyk, Inga, Author
Baidukova, Olga1, Author              
Skorb, Ekaterina. V.1, Author              
Affiliations:
1Katja Skorb (Indep. Res.), Biomaterialien, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_2231640              

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Free keywords: immobilization, layer-by-layer assembly, photochemistry, proteins, stimuli-responsive materials
 Abstract: A new principle of photo-assisted spatial desorption of (poly)histidine-tagged (His-Tag) proteins on a TiO2 surface is suggested. A semiconductor TiO2 surface is decorated by layer-by-layer (LbL) assembly of a strong polyelectrolyte, namely, polystyrene sulfonate (PSS), and nickel–nitrilotriacetic acid (NTA). The PSS/NTA multilayer architecture provides n-fold (n×NTA) binding efficiency for more precise protein recognition in comparison to existing molecular His-Tag protein recognition with one- and threefold multiplication (1×NTA, 3×NTA). Spatially resolved desorption of proteins is regulated by non- photodestructive short-term low-intensity light irradiation (<5 mW cm−2). The local pH shift on irradiated TiO2 selectively affects the pH-sensitive NTA/protein complex, but not the LbL assembly of PSS and NTA, which is stable in a broad pH range.

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 Dates: 2016-08-122016-09
 Publication Status: Published in print
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 Identifiers: DOI: 10.1002/celc.201600268
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Title: ChemElectroChem
  Abbreviation : ChemElectroChem
Source Genre: Journal
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Publ. Info: Weinheim, Germany : WILEY-VCH Verlag GmbH & Co. KGaA
Pages: - Volume / Issue: 3 (9) Sequence Number: - Start / End Page: 1306 - 1310 Identifier: ISSN: 2196-0216