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  Kinetics of spontaneous and EF-G-accelerated rotation of ribosomal subunits.

Sharma, H., Adio, S., Senyushkina, T., Belardinelli, R., Peske, F., & Rodnina, M. V. (2016). Kinetics of spontaneous and EF-G-accelerated rotation of ribosomal subunits. Cell Reports, 16(8), 2187-2196. doi:10.1016/j.celrep.2016.07.051.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002B-2427-3 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-1D4E-E
Genre: Journal Article

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 Creators:
Sharma, H.1, Author              
Adio, S.1, Author              
Senyushkina, T.1, Author              
Belardinelli, R.1, Author              
Peske, F.1, Author              
Rodnina, M. V.1, Author              
Affiliations:
1Department of Physical Biochemistry, MPI for biophysical chemistry, Max Planck Society, ou_578598              

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 Abstract: Ribosome dynamics play an important role in translation. The rotation of the ribosomal subunits relative to one another is essential for tRNA-mRNA translocation. An important unresolved question is whether subunit rotation limits the rate of translocation. Here, we monitor subunit rotation relative to peptide bond formation and translocation using ensemble kinetics and single-molecule FRET. We observe that spontaneous forward subunit rotation occurs at a rate of 40 s-1, independent of the rate of preceding peptide bond formation. Elongation factor G (EF-G) accelerates forward subunit rotation to 200 s-1. tRNA-mRNA movement is much slower (10-40 s-1), suggesting that forward subunit rotation does not limit the rate of translocation. The transition back to the non-rotated state of the ribosome kinetically coincides with tRNA-mRNA movement. Thus, large-scale movements of the ribosome are intrinsically rapid and gated by its ligands such as EF-G and tRNA.

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Language(s): eng - English
 Dates: 2016-08-112016-08-23
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1016/j.celrep.2016.07.051
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Title: Cell Reports
Source Genre: Journal
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Pages: - Volume / Issue: 16 (8) Sequence Number: - Start / End Page: 2187 - 2196 Identifier: -