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  Enhanced cross-species utility of conserved microsatellite markers in shorebirds

Küpper, C., Burke, T., Székely, T., & Dawson, D. A. (2008). Enhanced cross-species utility of conserved microsatellite markers in shorebirds. BMC Genomics, 9: 502. doi:10.1186/1471-2164-9-502.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002B-254C-B Version Permalink: http://hdl.handle.net/21.11116/0000-0002-EAC4-9
Genre: Journal Article

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Küpper, Clemens1, Author              
Burke, T., Author
Székely, T., Author
Dawson, D. A., Author
Affiliations:
1University of Bath, ou_persistent22              

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 Abstract: Background: Microsatellite markers are popular genetic markers frequently used in forensic biology. Despite their popularity, the characterisation of polymorphic microsatellite loci and development of suitable markers takes considerable effort. Newly-available genomic databases make it feasible to identify conserved genetic markers. We examined the utility and characteristics of conserved microsatellite markers in Charadriiformes (plovers, sandpipers, gulls and auks). This order harbours many species with diverse breeding systems, life histories and extraordinary migration biology whose genetics warrant investigation. However, research has been largely restrained by the limited availability of genetic markers. To examine the utility of conserved microsatellite loci as genetic markers we collated a database of Charadriiformes microsatellites, searched for homologues in the chicken genome and tested conserved markers for amplification and polymorphism in a range of charadriiform species. Results: Sixty-eight (42%) of 161 charadriiform microsatellite loci were assigned to a single location in the chicken genome based on their E-value. Fifty-five primers designed from conserved microsatellite loci with an E-value of E-10 or lower amplified across a wider range of charadriiform species than a control group of primers from ten anonymous microsatellite loci. Twenty-three of 24 examined conserved markers were polymorphic, each in on average 3 of 12 species tested. Conclusion: Genomic sequence databases are useful tools to identify conserved genetic markers including those located in non-coding regions. By maximising primer sequence similarity between source species and database species, markers can be further improved and provide additional markers to study the molecular ecology of populations of non-model organisms.

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 Dates: 2008
 Publication Status: Published in print
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 Rev. Method: -
 Identifiers: Other: WOS:000261238500001
DOI: 10.1186/1471-2164-9-502
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Title: BMC Genomics
Source Genre: Journal
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Publ. Info: BioMed Central
Pages: - Volume / Issue: 9 Sequence Number: 502 Start / End Page: - Identifier: ISSN: 1471-2164
CoNE: /journals/resource/111000136905010