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  Characterization of biosynthetic pathways for the production of the volatile homoterpenes DMNT and TMTT in Zea mays

Richter, A., Schaff, C., Zhang, Z., Lipka, A., Tian, F., Köllner, T. G., et al. (2016). Characterization of biosynthetic pathways for the production of the volatile homoterpenes DMNT and TMTT in Zea mays. The Plant Cell, 28(11), 2651-2665. doi:10.1105/tpc.15.00919.

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http://dx.doi.org/10.1105/tpc.15.00919 (Publisher version)
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 Creators:
Richter, A., Author
Schaff, C., Author
Zhang, Z., Author
Lipka, A.E, Author
Tian, F., Author
Köllner, Tobias G.1, Author              
Schnee, Christiane1, Author              
Preiss, S., Author
Irmisch, Sandra1, 2, Author              
Jander, G., Author
Boland, Wilhelm3, Author              
Gershenzon, Jonathan1, Author              
Buckler, E. S., Author
Degenhardt, J., Author
Affiliations:
1Department of Biochemistry, Prof. J. Gershenzon, MPI for Chemical Ecology, Max Planck Society, ou_421893              
2IMPRS on Ecological Interactions, MPI for Chemical Ecology, Max Planck Society, Jena, DE, ou_421900              
3Department of Bioorganic Chemistry, Prof. Dr. W. Boland, MPI for Chemical Ecology, Max Planck Society, ou_24028              

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 Abstract: Plant volatiles not only have multiple defense functions against herbivores, fungi, and bacteria, but also have been implicated in signaling within the plant and towards other organisms. Elucidating the function of individual plant volatiles will require more knowledge of their biosynthesis and regulation in response to external stimuli. By exploiting the variation of herbivore-induced volatiles among 26 maize (Zea mays) inbred lines, we conducted a nested association mapping (NAM) and genome-wide association study (GWAS) to identify a set of quantitative trait loci (QTLs) for investigating the pathways of volatile terpene production. The most significant identified QTL affects the emission of (E)-nerolidol, linalool, and the two homoterpenes, 3,8-dimethyl-1,4,7-nonatriene (DMNT), and (E,E)-4,8,12-trimethyltrideca-1,3,7,11-tetraene (TMTT). GWAS associated a single nucleotide polymorphism in the promoter of the gene encoding the terpene synthase TPS2 with this QTL. Biochemical characterization of TPS2 verified that this plastid-localized enzyme forms linalool, (E)-nerolidol, and (E,E)-geranyllinalool. The subsequent conversion of (E)-nerolidol into DMNT maps to a P450 monooxygenase, CYP92C5, which is capable of converting nerolidol into DMNT by oxidative degradation. A QTL influencing TMTT accumulation corresponds to a similar monooxygenase, CYP92C6, which is specific for the conversion of (E,E)-geranyllinalool to TMTT. The DMNT biosynthetic pathway and both monooxygenases are distinct from those previously characterized for DMNT and TMTT synthesis in Arabidopsis, suggesting independent evolution of these enzymatic activities.

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 Dates: 2016-082016-09-232016
 Publication Status: Published in print
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 Identifiers: Other: GER454
DOI: 10.1105/tpc.15.00919
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Title: The Plant Cell
  Abbreviation : Plant C
Source Genre: Journal
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Publ. Info: Rockville : American Society of Plant Physiologists
Pages: - Volume / Issue: 28 (11) Sequence Number: - Start / End Page: 2651 - 2665 Identifier: ISSN: 1532-298X
CoNE: https://pure.mpg.de/cone/journals/resource/1532-298X