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  Analysis of Enantioselective Biotransformations Using a Few Hundred Cells on an Integrated Microfluidic Chip

Krone, K. M., Warias, R., Ritter, C., Li, A., Acevedo-Rocha, C. G., Reetz, M. T., et al. (2016). Analysis of Enantioselective Biotransformations Using a Few Hundred Cells on an Integrated Microfluidic Chip. Journal of the American Chemical Society, 138(7), 2102-2105. doi:10.1021/jacs.5b12443.

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 Creators:
Krone, Karin, M.1, Author
Warias, Rico1, Author
Ritter, Cornelia2, Author
Li, Aitao2, 3, Author              
Acevedo-Rocha, Carlos G.2, 3, Author              
Reetz, Manfred T.2, 3, Author              
Belder, Detlev1, Author
Affiliations:
1Institute of Analytical Chemistry, University of Leipzig, Linnéstrasse 3, 04103 Leipzig, Germany, ou_persistent22              
2Faculty of Chemistry, Philipps-Universität Marburg, Hans-Meerwein-Strasse, 35032 Marburg, Germany, ou_persistent22              
3Research Department Reetz, Max-Planck-Institut für Kohlenforschung, Max Planck Society, ou_1445588              

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 Abstract: The investigation of stereoselective biocatalytic transformations at a single-cell level is to date an unsolved challenge. Here, we report the development of an integrated microfluidic device which enables the analytical characterization of enantioselective reactions at nanoliter scale by combining whole-cell catalyzed on-chip syntheses, chiral microchip electrophoresis, and label-free detection of enantiomers by deep UV time-resolved fluorescence. Using Escherichia coli expressing recombinant Aspergillus niger epoxide hydrolase as the model enzyme for various enantioselective reactions, we evaluated the approach for downscaling the reaction to a few hundred cells. Our work is thus an important step toward the analysis of single-cell stereoselective biocatalysis.

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Language(s): eng - English
 Dates: 2015-12-032016-02-092016-02-24
 Publication Status: Published in print
 Pages: 4
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1021/jacs.5b12443
 Degree: -

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Title: Journal of the American Chemical Society
  Other : J. Am. Chem. Soc.
  Abbreviation : JACS
Source Genre: Journal
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Publ. Info: Washington, DC : American Chemical Society
Pages: - Volume / Issue: 138 (7) Sequence Number: - Start / End Page: 2102 - 2105 Identifier: ISSN: 0002-7863
CoNE: https://pure.mpg.de/cone/journals/resource/954925376870