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  Learning lessons from directed evolution of stereoselective enzymes

Li, G., & Reetz, M. T. (2016). Learning lessons from directed evolution of stereoselective enzymes. Organic Chemistry Frontiers, 3(10), 1350-1358. doi:10.1039/C6QO00210B.

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 Creators:
Li, Guangyue1, Author              
Reetz, Manfred T.2, Author              
Affiliations:
1Research Department Reetz, Max-Planck-Institut für Kohlenforschung, Max Planck Society, ou_1445588              
2Philipps-Universität Marburg, Fachbereich Chemie, ou_persistent22              

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 Abstract: With the advent of directed evolution of stereoselective enzymes almost 20 years ago and the rapid development of this exciting area of research, the traditional limitations of biocatalysts in organic chemistry have been eliminated. It is now possible to enhance or invert enantioselectivity, broaden the substrate scope and increase the activity of many different types of enzymes. In addition to providing a prolific source of catalysts for asymmetric transformations, many lessons can be learned from directed evolution on the molecular level, because stereoselectivity is a sensitive probe. This review focuses on two types of lessons arising from studies focusing on (1) uncovering the source of altered stereoselectivity, and (2) constructing fitness landscapes which reveal additive and non-additive mutational effects as well as ways to escape from local minima. Case studies featuring enzymes of the type epoxide hydrolase, lipase and Baeyer–Villiger monooxygenase are presented.

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Language(s): eng - English
 Dates: 2016-06-27
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1039/C6QO00210B
 Degree: -

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Title: Organic Chemistry Frontiers
  Abbreviation : Org. Chem. Front.
Source Genre: Journal
 Creator(s):
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Publ. Info: Royal Society of Chemistry
Pages: - Volume / Issue: 3 (10) Sequence Number: - Start / End Page: 1350 - 1358 Identifier: ISSN: 2052-4129
CoNE: https://pure.mpg.de/cone/journals/resource/2052-4129