English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  A protein map of the yeast activated spliceosome as obtained by electron microscopy.

Sun, C., Rigo, N., Fabrizio, P., Kastner, B., & Lührmann, R. (2016). A protein map of the yeast activated spliceosome as obtained by electron microscopy. RNA, 22(9), 1427-1440. doi:10.1261/rna.057778.116.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002B-2DE6-2 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-87DD-5
Genre: Journal Article

Files

show Files
hide Files
:
2331909.pdf (Publisher version), 2MB
Name:
2331909.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2331909_Suppl_1.docx (Supplementary material), 15KB
Name:
2331909_Suppl_1.docx
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/vnd.openxmlformats-officedocument.wordprocessingml.document / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2331909_Suppl_2.tif (Supplementary material), 2MB
Name:
2331909_Suppl_2.tif
Description:
-
Visibility:
Public
MIME-Type / Checksum:
image/tiff / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2331909_Suppl_3.tif (Supplementary material), 5MB
Name:
2331909_Suppl_3.tif
Description:
-
Visibility:
Public
MIME-Type / Checksum:
image/tiff / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2331909_Suppl_4.tif (Supplementary material), 52MB
Name:
2331909_Suppl_4.tif
Description:
-
Visibility:
Public
MIME-Type / Checksum:
image/tiff / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2331909_Suppl_5.docx (Supplementary material), 16KB
Name:
2331909_Suppl_5.docx
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/vnd.openxmlformats-officedocument.wordprocessingml.document / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show
hide
Description:
-

Creators

show
hide
 Creators:
Sun, C.1, Author              
Rigo, N.1, Author              
Fabrizio, P.1, Author              
Kastner, B.1, Author              
Lührmann, R.1, Author              
Affiliations:
1Department of Cellular Biochemistry, MPI for biophysical chemistry, Max Planck Society, ou_578576              

Content

show
hide
Free keywords: B-act complex; Electron microscopy; Immunolabeling; Protein localization; Spliceosome
 Abstract: We have elucidated the spatial arrangement of proteins and snRNP subunits within the purified spliceosomal Bact complex from Saccharomyces cerevisiae, using negative-stain immunoelectron microscopy. The Bact spliceosome exhibits a mushroom-like shape with a main body connected to a foot and a steep and a shallow slope. The U5 core components, including proteins Snu114 and Prp8, are located in the main body and foot, while Brr2 is on the shallow slope. U2 snRNP components and the RNA helicase Prp2 were predominantly located in the upper regions of both slopes. While several proteins of the “nineteen complex” are located on the steep slope, Prp19, Cef1, and the U6 snRNA-binding protein Cwc2 are on the main body. Our results also indicate that the catalytic core RNP of the spliceosome resides in its main body. We thus assign distinct domains of the Bact complex to its snRNP and protein components, and we provide first structural insights into the remodeling events at the spliceosome during its transformation from the B to the Bact complex.

Details

show
hide
Language(s): eng - English
 Dates: 2016-07-012016-09
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1261/rna.057778.116
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: RNA
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 22 (9) Sequence Number: - Start / End Page: 1427 - 1440 Identifier: -