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  Smelling the difference: Transcriptome, proteome and volatilome changes after mating

Freihorst, D., Brunsch, M., Wirth, S., Krause, K., Kniemeyer, O., Linde, J., et al. (2018). Smelling the difference: Transcriptome, proteome and volatilome changes after mating. Fungal Genetics and Biology, 112, 2-11. doi:10.1016/j.fgb.2016.08.007.

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 Creators:
Freihorst, Daniela, Author
Brunsch, Melanie, Author
Wirth, Sophia, Author
Krause, Katrin, Author
Kniemeyer, Olaf, Author
Linde, Jörg, Author
Kunert, Maritta1, Author              
Boland, Wilhelm1, Author              
Kothe, Erika, Author
Affiliations:
1Department of Bioorganic Chemistry, Prof. Dr. W. Boland, MPI for Chemical Ecology, Max Planck Society, ou_24028              

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 Abstract: Mushrooms, such as Schizophyllum commune, have a specific odor. Whether this is linked to mating, prerequisite for mushroom formation, or also found in monokaryotic, unmated strains, was investigated with a comprehensive study on the transcriptome and proteome of this model organism. Mating interactions were investigated using a complete, cytosolic proteome map for unmated, monokaryotic, as well as for mated, dikaryotic mycelia. The regulations of the proteome were compared to transcriptional changes upon mating and to changes in smell by volatilome studies. We could show a good overlap between proteome and transcriptome data, but extensive posttranslational regulation was identified for more than 80 % of transcripts. This suggests down-stream regulation upon interaction of mating partners and formation of the dikaryon that is competent to form fruiting bodies. The volatilome was shown to respond to mating by a broader spectrum of volatiles and increased emission of the mushroom smell molecules 3-octanone and 1-octen-3-ol, as well as ethanol and ß-bisabolol in the dikaryon. Putatively involved biosynthetic proteins like alcohol dehydrogenases, Ppo-like oxygenases, or sesquiterpene synthases showed correlating transcriptional regulation depending on either mono- or dikaryotic stages.

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 Dates: 2016-09-012016-09-012018-03
 Publication Status: Published in print
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 Identifiers: Other: BOL661
DOI: 10.1016/j.fgb.2016.08.007
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Title: Fungal Genetics and Biology
  Other : Fungal Genet. Biol.
Source Genre: Journal
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Publ. Info: San Diego, CA : Academic Press
Pages: - Volume / Issue: 112 Sequence Number: - Start / End Page: 2 - 11 Identifier: ISSN: 1087-1845
CoNE: https://pure.mpg.de/cone/journals/resource/954926962932