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  Intracellular regulation of the insect chemoreceptor complex impacts odour localization in flying insects

Getahun, M. N., Thoma, M., Lavista Llanos, S., Keesey, I., Fandino, R. A., Knaden, M., et al. (2016). Intracellular regulation of the insect chemoreceptor complex impacts odour localization in flying insects. Journal of Experimental Biology, 219, 3428-3438. doi:10.1242/jeb.143396.

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 Creators:
Getahun, Merid Negash1, 2, Author              
Thoma, Michael1, 2, Author              
Lavista Llanos, Sofia1, Author              
Keesey, Ian1, Author              
Fandino, Richard A., Author
Knaden, Markus3, Author              
Wicher, Dieter1, Author              
Olsson, Shannon B.1, Author              
Hansson, Bill S.1, Author              
Affiliations:
1Department of Evolutionary Neuroethology, Prof. B. S. Hansson, MPI for Chemical Ecology, Max Planck Society, ou_421894              
2IMPRS on Ecological Interactions, MPI for Chemical Ecology, Max Planck Society, Jena, DE, ou_421900              
3Research Group Dr. M. Knaden, Insect Behavior, Department of Neuroethology, Prof. B. S. Hansson, MPI for Chemical Ecology, Max Planck Society, ou_421913              

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 Abstract: Flying insects are well known for airborne odour tracking and have evolved diverse chemoreceptors. While ionotropic receptors (IRs) are found across protostomes, insect odorant receptors (ORs) have only been identified in winged insects. We therefore hypothesized that the unique signal transduction of ORs offers an advantage for odour localization in flight. Using Drosophila, we found expression and increased activity of the intracellular signalling protein PKC in antennal sensilla following odour stimulation. Odour stimulation also enhanced phosphorylation of theORco-receptorOrco in vitro, while site-directed mutation of Orco or mutations in PKC subtypes reduced the sensitivity and dynamic range of OR-expressing neurons in vivo, but not IR-expressing neurons. We ultimately show that these mutations reduce competence for odour localization of flies in flight.We conclude that intracellular regulation of OR sensitivity is necessary for efficient odour localization, which suggests a mechanistic advantage for the evolution of the OR complex in flying insects.

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 Dates: 2016-082016-09-022016-11
 Publication Status: Published in print
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 Identifiers: Other: HAN277
DOI: 10.1242/jeb.143396
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Title: Journal of Experimental Biology
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Publ. Info: London : Published for the Company of Biologists Ltd. by the Cambridge University Press
Pages: - Volume / Issue: 219 Sequence Number: - Start / End Page: 3428 - 3438 Identifier: ISSN: 0022-0949
CoNE: https://pure.mpg.de/cone/journals/resource/110992357319088