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  The bicoid mRNA localization factor Exuperantia is an RNA-binding pseudonuclease.

Lazzaretti, D., Veith, K., Kramer, K., Basquin, C., Urlaub, H., Iron, U., et al. (2016). The bicoid mRNA localization factor Exuperantia is an RNA-binding pseudonuclease. Nature Structural and Molecular Biology, 23(8), 705-713. doi:10.1038/nsmb.3254.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002B-2F22-C Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-DE4D-F
Genre: Journal Article

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 Creators:
Lazzaretti, D., Author
Veith, K., Author
Kramer, K.1, Author              
Basquin, C., Author
Urlaub, H.1, Author              
Iron, U., Author
Bono, F., Author
Affiliations:
1Research Group of Bioanalytical Mass Spectrometry, MPI for biophysical chemistry, Max Planck Society, ou_578613              

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Free keywords: Developmental biology; RNA; RNA-binding proteins; X-ray crystallography
 Abstract: Anterior patterning in Drosophila is mediated by the localization of bicoid (bcd) mRNA at the anterior pole of the oocyte. Exuperantia (Exu) is a putative exonuclease (EXO) associated with bcd and required for its localization. We present the crystal structure of Exu, which reveals a dimeric assembly with each monomer consisting of a 3′-5′ EXO-like domain and a sterile alpha motif (SAM)-like domain. The catalytic site is degenerate and inactive. Instead, the EXO-like domain mediates dimerization and RNA binding. We show that Exu binds RNA directly in vitro, that the SAM-like domain is required for RNA binding activity and that Exu binds a structured element present in the bcd 3′ untranslated region with high affinity. Through structure-guided mutagenesis, we show that Exu dimerization is essential for bcd localization. Our data demonstrate that Exu is a noncanonical RNA-binding protein with EXO-SAM-like domain architecture that interacts with its target RNA as a homodimer.

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Language(s): eng - English
 Dates: 2016-07-042016-08
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1038/nsmb.3254
 Degree: -

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Title: Nature Structural and Molecular Biology
Source Genre: Journal
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Pages: - Volume / Issue: 23 (8) Sequence Number: - Start / End Page: 705 - 713 Identifier: -