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  Gossypol toxicity and detoxification in Helicoverpa armigera and Heliothis virescens

Krempl, C., Heidel-Fischer, H. M., Jimenez-Aleman, G. H., Reichelt, M., Menezes, R. C., Boland, W., et al. (2016). Gossypol toxicity and detoxification in Helicoverpa armigera and Heliothis virescens. Insect Biochemistry and Molecular Biology, 78, 69-77. doi:10.1016/j.ibmb.2016.09.003.

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 Creators:
Krempl, Corinna1, 2, Author              
Heidel-Fischer, Hanna M.1, Author              
Jimenez-Aleman, Guillermo Hugo2, 3, Author              
Reichelt, Michael4, Author              
Menezes, Riya Christina5, Author              
Boland, Wilhelm3, Author              
Vogel, Heiko1, Author              
Heckel, David G.1, Author              
Joussen, Nicole1, Author              
Affiliations:
1Department of Entomology, Prof. D. G. Heckel, MPI for Chemical Ecology, Max Planck Society, ou_421895              
2IMPRS on Ecological Interactions, MPI for Chemical Ecology, Max Planck Society, Jena, DE, ou_421900              
3Department of Bioorganic Chemistry, Prof. Dr. W. Boland, MPI for Chemical Ecology, Max Planck Society, ou_24028              
4Department of Biochemistry, Prof. J. Gershenzon, MPI for Chemical Ecology, Max Planck Society, ou_421893              
5Research Group Mass Spectrometry, MPI for Chemical Ecology, Max Planck Society, ou_421899              

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 Abstract: Gossypol is a polyphenolic secondary metabolite produced by cotton plants, which is toxic to many organisms. Gossypol's aldehyde groups are especially reactive, forming Schiff bases with amino acids of proteins and cross-linking them, inhibiting enzyme activities and contributing to toxicity. Very little is known about gossypol's mode of action and its detoxification in cotton-feeding insects that can tolerate certain concentrations of this compound. Here, we tested the toxicity of gossypol and a gossypol derivative lacking free aldehyde groups (SB-gossypol) toward Helicoverpa armigera and Heliothis virescens, two important pests on cotton plants. Larval feeding studies with these two species on artificial diet supplemented with gossypol or SB-gossypol revealed no detectable toxicity of gossypol, when the aldehyde groups were absent. A cytochrome P450 enzyme, CYP6AE14, is upregulated in H. armigera feeding on gossypol, and has been claimed to directly detoxify gossypol. However, using in vitro assays with heterologously expressed CYP6AE14, no metabolites of gossypol were detected, and further studies suggest that gossypol is not a direct substrate of CYP6AE14. Furthermore, larvae feeding on many other plant toxins also upregulate CYP6AE14. Our data demonstrate that the aldehyde groups are critical for the toxicity of gossypol when ingested by H. armigera and H. virescens larvae, and suggest that CYP6AE14 is not directly involved in gossypol metabolism, but may play a role in the general stress response of H. armigera larvae toward plant toxins.

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 Dates: 2016-09-232016-102016
 Publication Status: Published in print
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 Rev. Type: -
 Identifiers: Other: HEC347
DOI: 10.1016/j.ibmb.2016.09.003
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Title: Insect Biochemistry and Molecular Biology
  Other : Insect Biochem. Mol. Biol.
Source Genre: Journal
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Publ. Info: Oxford [England] : Pergamon
Pages: - Volume / Issue: 78 Sequence Number: - Start / End Page: 69 - 77 Identifier: ISSN: 0965-1748
CoNE: https://pure.mpg.de/cone/journals/resource/954925581163