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  A data set from flash X-ray imaging of carboxysomes

Hantke, M. F., Hasse, D., Ekeberg, T., John, K., Svenda, M., Loh, D., et al. (2016). A data set from flash X-ray imaging of carboxysomes. Scientific Data, 3: 160061. doi:10.1038/sdata.2016.61.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002B-30B4-F Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002B-30B5-D
Genre: Journal Article

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2016
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© M. F. Hantke et al.

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http://dx.doi.org/10.1038/sdata.2016.61 (Publisher version)
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 Creators:
Hantke, Max F.1, Author
Hasse, Dirk1, Author
Ekeberg, Tomas1, Author
John, Katja1, Author
Svenda, Martin1, Author
Loh, Duane2, Author
Martin, Andrew V.3, Author
Timneanu, Nicusor1, 4, Author
Larsson, Daniel S.D.1, Author
van der Schot, Gijs1, Author
Carlsson, Gunilla H.1, Author
Ingelman, Margareta1, Author
Andreasson, Jakob1, 5, Author
Westphal, Daniel1, Author
Iwan, Bianca1, Author
Uetrecht, Charlotte1, Author
Bielecki, Johan1, Author
Liang, Mengning6, Author
Stellato, Francesco6, 7, Author
DePonte, Daniel P.8, Author
Bari, Sadia9, 10, AuthorHartmann, Robert11, AuthorKimmel, Nils12, Author              Kirian, Richard A.6, AuthorSeibert, M. Marvin1, 8, AuthorMühlig, Kerstin1, AuthorSchorb, Sebastian8, AuthorFerguson, Ken8, AuthorBostedt, Christoph8, AuthorCarron, Sebastian8, AuthorBozek, John D.8, AuthorRolles, Daniel6, 13, 14, 15, Author              Rudenko, Artem13, 15, 16, Author              Foucar, Lutz14, 15, Author              Epp, Sascha W.15, 16, 17, Author              Chapman, Henry N.6, AuthorBarty, Anton6, AuthorAndersson, Inger1, AuthorHajdu, Janos1, 9, AuthorMaia, Filipe R.N.C.1, 18, Author more..
Affiliations:
1Department of Cell and Molecular Biology, Laboratory of Molecular Biophysics, Uppsala University, Husargatan 3 (Box 596), Uppsala SE-751 24, Sweden, ou_persistent22              
2Centre for BioImaging Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543, Singapore, ou_persistent22              
3ARC Centre of Excellence for Advanced Molecular Imaging, School of Physics, The University of Melbourne, Victoria 3010, Australia, ou_persistent22              
4Department of Physics and Astronomy, Uppsala University, Lägerhyddsvägen 1, Box 516, Uppsala SE- 751 20, Sweden , ou_persistent22              
5ELI beamlines, Institute of Physics, Academy of Sciences of the Czech Republic, Na Slovance 2, Prague 18221, Czech Republic , ou_persistent22              
6Center for Free-Electron Laser Science, DESY, Notkestrasse 85, Hamburg 22607, Germany , ou_persistent22              
7I.N.F.N. and Physics Department, University of Rome ‘Tor Vergata’, Via della Ricerca Scientifica 1, Rome 00133, Italy , ou_persistent22              
8LCLS, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA , ou_persistent22              
9European XFEL GmbH, Albert-Einstein-Ring 19, Hamburg 22761, Germany , ou_persistent22              
10Deutsches Elektronen-Synchrotron DESY, Notkestr. 85, Hamburg 22607, Germany , ou_persistent22              
11PNSensor GmbH, Römerstrasse 28, München 80803, Germany, ou_persistent22              
12High Energy Astrophysics, MPI for Extraterrestrial Physics, Max Planck Society, ou_159890              
13Department of Physics, J.R. Macdonald Laboratory, Kansas State University, Cardwell Hall, Manhattan, Kansas 66506, USA , ou_persistent22              
14Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              
15Max Planck Advanced Study Group at the Center for Free-Electron Laser Science (CFEL), Notkestraße 85, Hamburg 22607, Germany, ou_persistent22              
16Division Prof. Dr. Joachim H. Ullrich, MPI for Nuclear Physics, Max Planck Society, ou_904547              
17Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society, ou_1938288              
18NERSC, Lawrence Berkeley National Laboratory, Berkeley, California 94720, USA, ou_persistent22              

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Free keywords: Optics and photonics; Biophysics
 Abstract: Ultra-intense femtosecond X-ray pulses from X-ray lasers permit structural studies on single particles and biomolecules without crystals. We present a large data set on inherently heterogeneous, polyhedral carboxysome particles. Carboxysomes are cell organelles that vary in size and facilitate up to 40% of Earth’s carbon fixation by cyanobacteria and certain proteobacteria. Variation in size hinders crystallization. Carboxysomes appear icosahedral in the electron microscope. A protein shell encapsulates a large number of Rubisco molecules in paracrystalline arrays inside the organelle. We used carboxysomes with a mean diameter of 115±26 nm from Halothiobacillus neapolitanus. A new aerosol sample-injector allowed us to record 70,000 low-noise diffraction patterns in 12 min. Every diffraction pattern is a unique structure measurement and high-throughput imaging allows sampling the space of structural variability. The different structures can be separated and phased directly from the diffraction data and open a way for accurate, high-throughput studies on structures and structural heterogeneity in biology and elsewhere.

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Language(s): eng - English
 Dates: 2016-02-042016-06-012016-08-01
 Publication Status: Published online
 Pages: 8
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1038/sdata.2016.61
 Degree: -

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Title: Scientific Data
  Abbreviation : Sci. Data
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: London, United Kingdom : Nature Publishing Group
Pages: - Volume / Issue: 3 Sequence Number: 160061 Start / End Page: - Identifier: Other: 2052-4463
CoNE: /journals/resource/2052-4463