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  Fluorogenic probes for multicolor imaging in living cells.

Lukinavicius, G., Reymond, L., Umezawa, K., Sallin, O., D'Este, E., Göttfert, F., et al. (2016). Fluorogenic probes for multicolor imaging in living cells. Journal of the American Chemical Society, 138(30), 9365-9368. doi:10.1021/jacs.6b04782.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002B-3421-A Version Permalink: http://hdl.handle.net/21.11116/0000-0001-A4E7-1
Genre: Journal Article

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 Creators:
Lukinavicius, G.1, Author              
Reymond, L., Author
Umezawa, K., Author
Sallin, O., Author
D'Este, E.2, Author              
Göttfert, F.2, Author              
Ta, H.2, Author              
Hell, S. W.2, Author              
Urano, Y., Author
Johnsson, K., Author
Affiliations:
1Laboratory of Chromatin Labeling and Imaging, Max Planck Institute for Biophysical Chemistry, Max Planck Society, ou_2616691              
2Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society, ou_578627              

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 Abstract: Here we present a far-red, silicon-rhodamine-based fluorophore (SiR700) for live-cell multicolor imaging. SiR700 has excitation and emission maxima at 690 and 715 nm, respectively. SiR700-based probes for F-actin, microtubules, lysosomes, and SNAP-tag are fluorogenic, cell-permeable, and compatible with superresolution microscopy. In conjunction with probes based on the previously introduced carboxy-SiR650, SiR700-based probes permit multicolor live-cell superresolution microscopy in the far-red, thus significantly expanding our capacity for imaging living cells.

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Language(s): eng - English
 Dates: 2016-07-212016-08-03
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1021/jacs.6b04782
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Title: Journal of the American Chemical Society
Source Genre: Journal
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Pages: - Volume / Issue: 138 (30) Sequence Number: - Start / End Page: 9365 - 9368 Identifier: -