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  Actin- and myosin-dependent vesicle loading of presynaptic docking sites prior to exocytosis.

Miki, T., Malagon, G., Pulido, C., Llano, I., Neher, E., & Marty, A. (2016). Actin- and myosin-dependent vesicle loading of presynaptic docking sites prior to exocytosis. Neuron, 91(4), 808-823. doi:10.1016/j.neuron.2016.07.033.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002B-41D7-7 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002B-41DC-E
Genre: Journal Article

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 Creators:
Miki, T., Author
Malagon, G., Author
Pulido, C., Author
Llano, I., Author
Neher, E.1, Author              
Marty, A., Author
Affiliations:
1Emeritus Group of Membrane Biophysics, MPI for Biophysical Chemistry, Max Planck Society, ou_1571137              

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 Abstract: Variance analysis of postsynaptic current amplitudes suggests the presence of distinct docking sites (also called release sites) where vesicles pause before exocytosis. Docked vesicles participate in the readily releasable pool (RRP), but the relation between docking site number and RRP size remains unclear. It is also unclear whether all vesicles of the RRP are equally release competent, and what cellular mechanisms underlie RRP renewal. We address here these questions at single glutamatergic synapses, counting released vesicles using deconvolution. We find a remarkably low variance of cumulative vesicle counts during action potential trains. This, combined with Monte Carlo simulations, indicates that vesicles transit through two successive states before exocytosis, so that the RRP is up to 2-fold higher than the docking site number. The transition to the second state has a very rapid rate constant, and is specifically inhibited by latrunculin B and blebbistatin, suggesting the involvement of actin and myosin.

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Language(s): eng - English
 Dates: 2016-08-17
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1016/j.neuron.2016.07.033
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Title: Neuron
Source Genre: Journal
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Pages: - Volume / Issue: 91 (4) Sequence Number: - Start / End Page: 808 - 823 Identifier: -