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  Kidins220/ARMS binds to the B cell antigen recptor and regulates B cell development and activation

Fiala, G. J., Janowska, I., Prutek, F., Hobeika, E., Satapathy, A., Sprenger, A., et al. (2015). Kidins220/ARMS binds to the B cell antigen recptor and regulates B cell development and activation. The Journal of Experimental Medicine, 212(10), 1693-1708.

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 Creators:
Fiala, Gina J.1, 2, 3, 4, Author              
Janowska, Iga1, 3, 5, Author
Prutek, F.1, 3, 4, Author              
Hobeika, E.3, 4, 6, Author              
Satapathy, Annyesha7, Author
Sprenger, Adrian1, 8, 9, Author
Plum, Thomas1, 3, 5, Author
Seidl, Maximilian3, 10, Author
Dengjel, Jörn1, 8, 9, Author
Reth, Michael1, 4, Author              
Cesca, Fabrizia7, Author
Brummer, Tilman1, 11, 12, Author
Minguet, Susanna3, 4, Author              
Schamel, Wolfgang W. A.1, 3, 4, Author              
Affiliations:
1Centre for Biological Signaling Studies (BIOSS), University of Freiburg, Freiburg, Germany, ou_persistent22              
2Spemann Graduate School of Biology and Medicine (SGBM), University of Freiburg, Freiburg, Germany, ou_persistent22              
3Centre of Chronic Immunodeficiency, (CCI), University of Freiburg, Freiburg, Germany, ou_persistent22              
4Research Group and Chair of Molecular Immunology of the University of Freiburg, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243645              
5Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, 79108 Freiburg, DE, ou_2243640              
6Institute of Immunology, University Hospital Ulm, Ulm, Germany, ou_persistent22              
7Center of Synaptic Neuroscience, Italian Institute of Technology, Genova, Italy, ou_persistent22              
8Department of Dermatology, University of Freiburg, Freiburg, Germany, ou_persistent22              
9Center for Biological Systems Analaysis (ZBSA), University of Freiburg, Freiburg, Germany, ou_persistent22              
10Institute of Pathology, University Medical Centre Freiburg, Freiburg, Germany, ou_persistent22              
11Institute of Molecular Medicine and Cell Research, University of Freiburg, Freiburg, Germany, ou_persistent22              
12Comprehensive Cancer Centre Freiburg, University of Freiburg, Freiburg, Germany, ou_persistent22              

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 Abstract: B cell antigen receptor (BCR) signaling is critical for B cell development and activation. Using mass spectrometry, we identified a protein kinase D-interacting substrate of 220 kD (Kidins220)/ankyrin repeat-rich membrane-spanning protein (ARMS) as a novel interaction partner of resting and stimulated BCR. Upon BCR stimulation, the interaction increases in a Src kinase-independent manner. By knocking down Kidins220 in a B cell line and generating a conditional B cell-specific Kidins220 knockout (B-KO) mouse strain, we show that Kidins220 couples the BCR to PLCγ2, Ca(2+), and extracellular signal-regulated kinase (Erk) signaling. Consequently, BCR-mediated B cell activation was reduced in vitro and in vivo upon Kidins220 deletion. Furthermore, B cell development was impaired at stages where pre-BCR or BCR signaling is required. Most strikingly, λ light chain-positive B cells were reduced sixfold in the B-KO mice, genetically placing Kidins220 in the PLCγ2 pathway. Thus, our data indicate that Kidins220 positively regulates pre-BCR and BCR functioning.

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Language(s): eng - English
 Dates: 2015-08-31
 Publication Status: Published in print
 Pages: 16
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: -
 Degree: -

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Title: The Journal of Experimental Medicine
Source Genre: Journal
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Publ. Info: Rockefeller University Press
Pages: - Volume / Issue: 212 (10) Sequence Number: - Start / End Page: 1693 - 1708 Identifier: -