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  Influence of Core β-1,2-Xylosylation on Glycoprotein Recognition by Murine C-type Lectin Receptors and Its Impact on Dendritic Cell Targeting

Brzezicka, K., Vogel, U., Serna, S., Johannssen, T., Lepenies, B., & Reichardt, N.-C. (2016). Influence of Core β-1,2-Xylosylation on Glycoprotein Recognition by Murine C-type Lectin Receptors and Its Impact on Dendritic Cell Targeting. ACS Chemical Biology, 11(8), 2347-2356. doi:10.1021/acschembio.6b00265.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002B-45C7-2 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002B-45C8-F
Genre: Journal Article

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 Creators:
Brzezicka, Katarzyna, Author
Vogel, Uwe1, Author              
Serna, Sonia, Author
Johannssen, Timo1, Author              
Lepenies, Bernd1, Author              
Reichardt, Niels-Christian, Author
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1Bernd Lepenies, Biomolekulare Systeme, Max Planck Institute of Colloids and Interfaces, Max Planck Society, ou_1863298              

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 Abstract: Targeting antigens to dendritic cell subsets is a promising strategy to enhance the efficacy of vaccines. C-type lectin receptors (CLRs) expressed by dendritic cells are particularly attractive candidates since CLR engagement may promote cell uptake and may further stimulate antigen presentation and subsequent T cell activation. While most previous approaches have involved antibody-mediated CLR-targeting, glycan-based CLR targeting has become more and more attractive in recent years. In the present study, we show that small structural glycan modifications may markedly influence CLR recognition, dendritic cell targeting, and subsequent T cell activation. A biantennary N-glycan (G0) and its analogous O-2 core xylosylated N-glycan (XG0) were synthesized, covalently conjugated to the model antigen ovalbumin, and analyzed for binding to a set of murine CLR-Fc fusion proteins using lectin microarray. To evaluate whether the differential binding of G0 and XG0 to CLRs impacted dendritic cell targeting, uptake studies using murine dendritic cells were performed. Finally, effects of the ovalbumin glycoconjugates on T cell activation were measured in a dendritic cell/T cell cocultivation assay. Our results highlight the utility of glycan-based dendritic cell targeting and demonstrate that small structural differences may have a major impact on dendritic cell targeting efficacy.

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 Dates: 2016-06-172016-08-19
 Publication Status: Published in print
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 Identifiers: DOI: 10.1021/acschembio.6b00265
BibTex Citekey: doi:10.1021/acschembio.6b00265
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Title: ACS Chemical Biology
Source Genre: Journal
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Publ. Info: Washington, D.C. : American Chemical Society
Pages: - Volume / Issue: 11 (8) Sequence Number: - Start / End Page: 2347 - 2356 Identifier: ISSN: 1554-8929