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  Mechanism of the activation of proteinase inhibitor synthesis by systemin involves β-sheet structure, a specific DNA-binding protein domain

Slósarek, G., Kalbitzer, H. R., Mucha, P., Rekowski, P., Kupryszewski, G., Giel-Pietraszuk, M., et al. (1995). Mechanism of the activation of proteinase inhibitor synthesis by systemin involves β-sheet structure, a specific DNA-binding protein domain. Journal of Structural Biology, 115(1), 30-36. doi:10.1006/jsbi.1995.1026.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-002B-4FE0-1 Version Permalink: https://hdl.handle.net/11858/00-001M-0000-002B-4FE1-0
Genre: Journal Article
Alternative Title : Mechanism of the activation of proteinase inhibitor synthesis by systemin involves beta-sheet structure, a specific DNA-binding protein domain

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JStructBiol_115_1995_30.pdf (Any fulltext), 457KB
 
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 Creators:
Slósarek, Genowefa1, Author           
Kalbitzer, Hans Robert2, Author           
Mucha, Piotr, Author
Rekowski, Piotr, Author
Kupryszewski, Gotfryd, Author
Giel-Pietraszuk, Malgorzata, Author
Szymanski, Maciej, Author
Barciszewski, Jan, Author
Affiliations:
1Max Planck Institute for Medical Research, Max Planck Society, ou_1125545              
2Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497712              

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 Abstract: We analyzed a tertiary structure of systemin, the first identified polypeptide plant hormone, using two-dimensional NMR spectroscopy. From these data and molecular dynamics calculations we concluded that the peptide can adopt a Z-like-β-sheet structure, which has previously been found in many specific DNA-binding proteins. Using DNA-cellulose affinity chromatography, we showed that systemin binds strongly to DNA. We suggest that the specific systemin-DNA interaction, particularly in a promoter region of the proteinase inhibitors, could effect gone expression and thus explain the biological activity of systemin.

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Language(s): eng - English
 Dates: 1995-02-211995-06-031995-07-01
 Publication Status: Issued
 Pages: 7
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 665405
DOI: 10.1006/jsbi.1995.1026
URI: http://www.sciencedirect.com/science/article/pii/S104784778571026X
Other: 6611
 Degree: -

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Title: Journal of Structural Biology
  Abbreviation : J. Struct. Biol.
Source Genre: Journal
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Publ. Info: Orlando, Fla. : Academic Press
Pages: - Volume / Issue: 115 (1) Sequence Number: - Start / End Page: 30 - 36 Identifier: ISSN: 1047-8477
CoNE: https://pure.mpg.de/cone/journals/resource/954922650160