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  The use of luciferase as a reporter for response of plant cells to the fireblight pathogen Erwinia amylovora

Chang, J.-R., & Geider, K. (1995). The use of luciferase as a reporter for response of plant cells to the fireblight pathogen Erwinia amylovora. Plant Cell Reports, 14(8), 497-500. doi:10.1007/BF00232782.

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Datensatz-Permalink: https://hdl.handle.net/11858/00-001M-0000-002B-530E-2 Versions-Permalink: https://hdl.handle.net/11858/00-001M-0000-002B-530F-F
Genre: Zeitschriftenartikel
Alternativer Titel : The use of luciferase as a reporter for response of plant cells to the fireblight pathogen Erwinia amylovora

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PlantCellReports_14_1995_497.pdf (beliebiger Volltext), 422KB
 
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PlantCellReports_14_1995_497.pdf
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http://link.springer.com/content/pdf/10.1007%2FBF00232782.pdf (beliebiger Volltext)
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https://dx.doi.org/10.1007/BF00232782 (beliebiger Volltext)
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 Urheber:
Chang, Jeong-Rahn1, Autor           
Geider, Klaus1, Autor           
Affiliations:
1Research Group Prof. Dr. Geider, Max Planck Institute for Medical Research, Max Planck Society, ou_1497738              

Inhalt

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Schlagwörter: -
 Zusammenfassung: We have introduced the gene encoding luciferase from Photinus pyralis into pear and tobacco cells in order to judge the reaction of plant tissue to damaging conditions such as incubation at high temperature or inoculation with a pathogen. The constitutive expression of the luciferase gene via a strong promoter slowly decreased during propagation of the transformed pear cell line. After various stress treatments the resulting luciferase activity and the ATP content of the plant cells were determined by bioluminescence and found to correspond to each other. Inoculation of transformed pear cells with Erwinia amylovora resulted in a continuous decrease of luciferase activity in contrast to tobacco cells, where the enzyme activity was significantly higher in the first period after inoculation with bacteria compared to the untreated control cells. The pattern of the luciferase activity reflected the slow damage of the host-plant cells by E. amylovora and the elevated metabolism of the non-host cells after inoculation with the pathogen.

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Sprache(n): eng - English
 Datum: 1994-11-101994-09-191994-11-101995-05-08
 Publikationsstatus: Erschienen
 Seiten: 4
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: eDoc: 665205
DOI: 10.1007/BF00232782
URI: http://www.ncbi.nlm.nih.gov/pubmed/24185519
URI: http://link.springer.com/article/10.1007%2FBF00232782
Anderer: 6873
 Art des Abschluß: -

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Quelle 1

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Titel: Plant Cell Reports
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: Berlin : Springer
Seiten: - Band / Heft: 14 (8) Artikelnummer: - Start- / Endseite: 497 - 500 Identifikator: ISSN: 0721-7714
CoNE: https://pure.mpg.de/cone/journals/resource/954925532004