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  Structure of a Cytoplasmic 11-Subunit RNA Exosome Complex

Kowalinski, E., Kögel, A., Ebert, J., Reichelt, P., Stegmann, E., Habermann, B., et al. (2016). Structure of a Cytoplasmic 11-Subunit RNA Exosome Complex. Molecular Cell, 63(1), 125-134. doi:10.1016/j.molcel.2016.05.028.

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2016 The Author(s). Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license
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 Creators:
Kowalinski, Eva1, Author              
Kögel, Alexander1, Author              
Ebert, Judith1, Author              
Reichelt, Peter1, Author              
Stegmann, Elisabeth1, Author              
Habermann, Bianca2, Author              
Conti, Elena1, Author              
Affiliations:
1Conti, Elena / Structural Cell Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565144              
2Habermann, Bianca / Computational Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1832284              

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Free keywords: QUALITY-CONTROL SYSTEMS; MESSENGER-RNA; SKI COMPLEX; MACROMOLECULAR ASSEMBLIES; SACCHAROMYCES-CEREVISIAE; TRANSLATION ELONGATION; EUKARYOTIC EXOSOME; CRYSTAL-STRUCTURE; NUCLEAR EXOSOME; CENTRAL CHANNELBiochemistry & Molecular Biology; Cell Biology;
 Abstract: The RNA exosome complex associates with nuclear and cytoplasmic cofactors to mediate the decay, surveillance, or processing of a wide variety of transcripts. In the cytoplasm, the conserved core of the exosome (Exo10) functions together with the conserved Ski complex. The interaction of S. cerevisiae Exo10 and Ski is not direct but requires a bridging cofactor, Ski7. Here, we report the 2.65 angstrom resolution structure of S. cerevisiae Exo10 bound to the interacting domain of Ski7. Extensive hydrophobic interactions rationalize the high affinity and stability of this complex, pointing to Ski7 as a constitutive component of the cytosolic exosome. Despite the absence of sequence homology, cytoplasmic Ski7 and nuclear Rrp6 bind Exo(10) using similar surfaces and recognition motifs. Knowledge of the interacting residues in the yeast complexes allowed us to identify a splice variant of human HBS1-Like as a Ski7-like exosome-binding protein, revealing the evolutionary conservation of this cytoplasmic cofactor.

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Language(s): eng - English
 Dates: 2016-02-082016-05-182016-06-232016-07-07
 Publication Status: Published in print
 Pages: 10
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Project name : ERC Advanced Investigator Grant 294371
Grant ID : 294371
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Title: Molecular Cell
Source Genre: Journal
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Publ. Info: Cambridge, Mass. : Cell Press
Pages: - Volume / Issue: 63 (1) Sequence Number: - Start / End Page: 125 - 134 Identifier: ISSN: 1097-2765
CoNE: https://pure.mpg.de/cone/journals/resource/954925610929