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  Ionic interactions and the global conformations of the hammerhead ribozyme

Bassi, G. S., Møllegard, N.-E., Murchie, A. I. H., Von Kitzing, E., & Lilley, D. M. J. (1995). Ionic interactions and the global conformations of the hammerhead ribozyme. Nature Structural and Molecular Biology, 2(1), 45-55. doi:10.1038/nsb0195-45.

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Genre: Journal Article
Alternative Title : Ionic interactions and the global conformations of the hammerhead ribozyme

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NatStructBiol_2_1995_45.pdf (Any fulltext), 2MB
 
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 Creators:
Bassi, Gurminder S, Author
Møllegard, Niels-Erik, Author
Murchie, Alastair I. H., Author
Von Kitzing, Eberhard1, Author              
Lilley, David M. J., Author
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1Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497701              

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 Abstract: Here we investigate the global conformation of the hammerhead ribozyme. Electrophoretic studies demonstrate that the structure is folded in response to the concentration and type of ions present. Folding based on colinear alignment of arms II and III is suggested, with a variable angle subtended by the remaining helix I. In the probable active conformation, a small angle is subtended between helices I and II. Using uranyl photocleavage, an ion binding site has been detected in the long single-stranded region. The folded conformation could generate a preactivation of the scissile bond to permit in-line attack of the 2'-hydroxyl group, with a bound metal ion playing an integral role in the chemistry.

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Language(s): eng - English
 Dates: 1994-09-121994-12-161995-01-01
 Publication Status: Published in print
 Pages: 11
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
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Title: Nature Structural and Molecular Biology
  Other : Nature Struct Biol
Source Genre: Journal
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Publ. Info: New York, NY : Nature Pub. Group
Pages: - Volume / Issue: 2 (1) Sequence Number: - Start / End Page: 45 - 55 Identifier: ISSN: 1545-9993
CoNE: https://pure.mpg.de/cone/journals/resource/954925603763