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  Shedding light on biofilm formation of Halobacterium salinarum R1 by SWATH-LC/MS/MS analysis of planktonic and sessile cells.

Losensky, G., Jung, K., Urlaub, H., Pfeifer, F., Fröls, S., & Lenz, C. (2017). Shedding light on biofilm formation of Halobacterium salinarum R1 by SWATH-LC/MS/MS analysis of planktonic and sessile cells. Proteomics, 17(7): 1600111. doi:10.1002/pmic.201600111.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002B-5792-5 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-78BF-9
Genre: Journal Article

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 Creators:
Losensky, G., Author
Jung, K., Author
Urlaub, H.1, Author              
Pfeifer, F., Author
Fröls, S., Author
Lenz, C.1, Author              
Affiliations:
1Research Group of Bioanalytical Mass Spectrometry, MPI for biophysical chemistry, Max Planck Society, ou_578613              

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Free keywords: Archaea; Biofilm; Haloarchaea; Microbiology; SWATH
 Abstract: Early and mature biofilm formation in the extremely halophilic euryarchaeon Halobacterium salinarum strain R1 was characterized by SWATH-LC/MS/MS. Using a simple surfactant-assisted protein solubilization protocol and one-dimensional ultra-high performance nanoflow chromatography on the front end, 63.2% and 58.6% of the predicted Hbt. salinarum R1 proteome could be detected and quantified, respectively. Analysis of biophysical protein properties, functional analysis and pathway mapping indicated comprehensive characterization of the proteome. Sixty point eight percent of the quantified proteins (or 34.5% of the predicted proteome) exhibited significant abundance changes between planktonic and sessile states, demonstrating that haloarchaeal biofilm formation represents a profound "lifestyle change" on the molecular level. Our results and analysis constitute the first comprehensive study to track molecular changes from planktonic cultures to initial and mature archaeal biofilms on the proteome level. Data are available via ProteomeXchange, identifier PXD003667. Proteins exemplifying different protein expression level profiles were selected, and their corresponding gene transcripts targeted by qRT-PCR to test the feasibility of establishing rapid PCR-based assays for archaeal biofilm formation.

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Language(s): eng - English
 Dates: 2016-10-172017-04
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1002/pmic.201600111
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Title: Proteomics
Source Genre: Journal
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Pages: 13 Volume / Issue: 17 (7) Sequence Number: 1600111 Start / End Page: - Identifier: -