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  STED nanoscopy with wavelengths at the emission maximum.

Bordenave, M. D., Balzarotti, F., Stefani, F. D., & Hell, S. W. (2016). STED nanoscopy with wavelengths at the emission maximum. Journal of Physics D, 49(36): 365102. doi:10.1088/0022-3727/49/36/365102.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002B-5CE2-5 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002C-924F-D
Genre: Journal Article

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Bordenave, M. D., Author
Balzarotti, F., Author
Stefani, F. D., Author
Hell, S. W.1, Author              
Affiliations:
1Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society, ou_578627              

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 Abstract: Commonly, in stimulated emission depletion (STED) fluorescence nanoscopy, light of a wavelength located at the red tail of the emission spectrum of the dye is used to shrink the effective fluorophore excitation volume and thus to obtain images with sub diffraction resolution. Here, we demonstrate that continuous wave (CW) STED nanoscopy is feasible using STED wavelengths located at the emission maximum, where the cross section for stimulated emission is up to 10-fold larger than at the red tail. As a result, STED imaging becomes possible at equally lower STED beam power. Besides, fluorophores that have been considered inapplicable in certain wavelength constellations are thus becoming usable.

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Language(s): eng - English
 Dates: 2016-08-092016-09-14
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1088/0022-3727/49/36/365102
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Title: Journal of Physics D
Source Genre: Journal
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Pages: 8 Volume / Issue: 49 (36) Sequence Number: 365102 Start / End Page: - Identifier: -