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  Block of native Ca2+-permeable AMPA receptors in rat brain by intracellular polyamines generates double rectification

Koh, D. S., Burnashev, N., & Jonas, P. (1995). Block of native Ca2+-permeable AMPA receptors in rat brain by intracellular polyamines generates double rectification. The Journal of Physiology - London, 486(2), 305-312. doi:10.1113/jphysiol.1995.sp021020.

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Genre: Journal Article
Alternative Title : Block of native Ca2+-permeable AMPA receptors in rat brain by intracellular polyamines generates double rectification

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JPhysiol_486_1995_305.pdf (Any fulltext), 2MB
 
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http://www.ncbi.nlm.nih.gov/pmc/articles/PMC1156754/ (Supplementary material)
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 Creators:
Koh, Duk Su1, Author              
Burnashev, Nail1, Author              
Jonas, Peter1, Author              
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1Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497701              

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 Abstract: 1. The influence of intracellular factors on current rectification of different subtypes of native alpha-amino-3-hydroxy-5-methyl-4- isoxazoleproprionate receptors (AMPARs) was studied in rat brain slices by combining fast application of glutamate with patch pipette perfusion. 2. The peak current-voltage (I-V) relation of the AMPARs expressed in Bergmann glial cells of cerebellum and dentate gyrus (DG) basket cells of hippocampus was weakly rectifying in outside-out patches and nystatin-perforated vesicles, but showed a doubly rectifying shape with a region of reduced slope between 0 and +40 mV in nucleated patches. The I-V relation of AMPARs expressed in hippocampal CA3 pyramidal neurones was linear in all recording configurations. 3. Intracellular application of 25 microM spermine, a naturally occurring polyamine, blocked outward currents in outside-out patches from Bergmann glial cells and DG basket cells in a voltage-dependent manner, generating I-V relations with a doubly rectifying shape which were similar to those recorded in nucleated patches. AMPARs in CA3 pyramidal cell patches were unaffected by 25 microM spermine. 4. The half-maximal blocking concentration of spermine at +40 mV was 0.3 microM in Bergmann glial cell patches and 1.5 microM in DG basket cell patches, whereas it was much higher (>> 100 microM) for CA3 pyramidal cell patches. Spermidine also affected current rectification, but with lower affinity. The block of outward current by polyamines following voltage jumps developed within < 0.5 ms. 5. We conclude that current rectification, rather than being an intrinsic property of the Ca(2+)-permeable AMPAR channel, is generated by polyamine block.

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Language(s): eng - English
 Dates: 1994-04-201995-05-241995-11-01
 Publication Status: Published in print
 Pages: 9
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 Rev. Type: Peer
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Title: The Journal of Physiology - London
Source Genre: Journal
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Publ. Info: London : Cambridge University Press
Pages: - Volume / Issue: 486 (2) Sequence Number: - Start / End Page: 305 - 312 Identifier: ISSN: 0022-3751
CoNE: https://pure.mpg.de/cone/journals/resource/954925334693_2