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  Differential expression patterns of five acetylcholine receptor subunit genes in rat muscle during development

Kues, W., Sakmann, B., & Witzemann, V. (1995). Differential expression patterns of five acetylcholine receptor subunit genes in rat muscle during development. European Journal of Neuroscience: European Neuroscience Association, 7(6), 1376-1385. doi:10.1111/j.1460-9568.1995.tb01129.x.

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Genre: Journal Article
Alternative Title : Differential expression patterns of five acetylcholine receptor subunit genes in rat muscle during development

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EurJNeurosci_7_1995_1376.pdf (Any fulltext), 3MB
 
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 Creators:
Kues, Winfried1, Author              
Sakmann, Bert1, Author              
Witzemann, Veit1, 2, Author              
Affiliations:
1Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497701              
2Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497704              

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Free keywords: mRNA localization, acetylcholine receptor, myogenesis, transcript gradients
 Abstract: The spatial and temporal expression patterns of five genes which encode the α-, β-, γ-, δ- and ε-subunits of the nicotinic acetylcholine receptor in skeletal muscle were followed during development in the rat by in situ hybridization analysis. Three major developmental phases, characterized by specific expression patterns, could be distinguished. (i) During myogenic differentiation α-, β-, γ- and δ-subunit genes are activated and transcripts are expressed in muscle precursor cells at embryonic day 12 (E12) and during subsequent cell fusion. (ii) Following innervation of myotubes at approximately E15-E17 the mRNA of the α-, β-, γ-and δ-subunit genes accumulate in synaptic and decrease in extrasynaptic fibre regions during early synaptogenesis. The mRNA of the epsilon-subunit gene becomes detectable first in subsynaptic nuclei 2-3 days after innervation has occurred. (iii) During postnatal development α-, β- and δ-subunit transcript levels are reduced predominantly in extrasynaptic fibre segments and show significant differences in distribution depending on the muscle subtype whereas the γ-subunit mRNA disappears completely within the first postnatal week in all muscles. In contrast, the γ-subunit gene is transcribed only in subsynaptic myonuclei throughout development and in the adult muscle.

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Language(s): eng - English
 Dates: 1995-06-01
 Publication Status: Published in print
 Pages: 10
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: European Journal of Neuroscience : European Neuroscience Association
  Other : Eur. J. Neurosci
Source Genre: Journal
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Publ. Info: Oxford, UK : Published on behalf of the European Neuroscience Association by Oxford University Press
Pages: - Volume / Issue: 7 (6) Sequence Number: - Start / End Page: 1376 - 1385 Identifier: ISSN: 0953-816X
CoNE: https://pure.mpg.de/cone/journals/resource/954925575988