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  The exocytotic fusion pore of small granules has a conductance similar to an ion channel

Lollike, K., Borregaard, N., & Lindau, M. (1995). The exocytotic fusion pore of small granules has a conductance similar to an ion channel. Journal of Cell Biology, 129(1), 99-104. doi:10.1083/jcb.129.1.99.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-002B-6350-B Version Permalink: https://hdl.handle.net/11858/00-001M-0000-002B-74A9-B
Genre: Journal Article
Alternative Title : The exocytotic fusion pore of small granules has a conductance similar to an ion channel

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JCellBiol_129_1995_99.pdf (Any fulltext), 708KB
 
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 Creators:
Lollike, Karsten, Author
Borregaard, Niels, Author
Lindau, Manfred1, Author           
Affiliations:
1Department of Molecular Cell Research, Max Planck Institute for Medical Research, Max Planck Society, ou_1497703              

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 Abstract: We measured capacitance changes in cell attached patches of human neutrophils using a high frequency lock-in method. With this technique the noise level is reduced to 0.025 fF such that capacitance steps of 0.1 fF are clearly detected corresponding to exo- and endocytosis of single 60 nm vesicles. It is thus possible to detect almost all known exocytotic and endocytotic processes including exocytosis of small neurotransmitter containing vesicles in most cell types as well as endocytosis of coated and uncoated pits. In neutrophils we demonstrate a stepwise capacitance decrease generated by 60-165 nm vesicles as expected for endocytosis of coated and non-coated pits. Following ionomycin stimulation a stepwise capacitance increase is observed consisting of 0.1-5 fF steps corresponding to the different granule types of human neutrophils from secretory vesicles to azurophil granules. The opening of individual fusion pores is resolved during exocytosis of 200 nm vesicles. The initial conductance has a mean value of 150 pS and can be as low as 35 pS which is similar to the conductance of many ion channels suggesting that the initial fusion pore is formed by a protein complex.

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Language(s): eng - English
 Dates: 1994-11-071994-09-081994-11-071995-04-01
 Publication Status: Issued
 Pages: 6
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 665208
DOI: 10.1083/jcb.129.1.99
URI: http://www.jcb.org/cgi/content/abstract/129/1/99
URI: http://www.ncbi.nlm.nih.gov/pubmed/7535305
Other: 6868
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Title: Journal of Cell Biology
Source Genre: Journal
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Publ. Info: New York, NY : Rockefeller Institute Press
Pages: - Volume / Issue: 129 (1) Sequence Number: - Start / End Page: 99 - 104 Identifier: ISSN: 0021-9525
CoNE: https://pure.mpg.de/cone/journals/resource/991042742946024_1