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  Docked granules, the exocytic burst, and the need for ATP hydrolysis in endocrine cells

Parsons, T. D., Coorssen, J. R., Horstmann, H., Horstmann, H., & Almers, W. (1995). Docked granules, the exocytic burst, and the need for ATP hydrolysis in endocrine cells. Neuron, 15(5), 1085-1096. doi:10.1016/0896-6273(95)90097-7.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002B-7270-C Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002B-7271-A
Genre: Journal Article
Alternative Title : Docked granules, the exocytic burst, and the need for ATP hydrolysis in endocrine cells

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 Creators:
Parsons, Thomas D.1, Author              
Coorssen, J. R.1, Author              
Horstmann, Heinz2, Author              
Horstmann, Heinz1, Author              
Almers, Wolfhard1, Author              
Affiliations:
1Department of Molecular Cell Research, Max Planck Institute for Medical Research, Max Planck Society, ou_1497703              
2Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497699              

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 Abstract: Ca(2+)-triggered exocytosis was studied in single rat melanotrophs and bovine chromaffin cells by capacitance measurements. Sustained exocytosis required MgATP, but even in the absence of MgATP, Ca2+ could trigger exocytosis of 2700 granules in a typical melanotroph and of 840 granules in a chromaffin cell. Granules undergoing ATP-independent exocytosis were similar in number to those appearing docked to the plasmalemma in quickly frozen unfixed sections (3300 in a melanotroph and 830 in a chromaffin cell). Most exocytosis required tens of seconds, but a small pool of granules was released in tens of milliseconds. Evidently, only a small subset of docked granules is rapidly releasable. We suggest that, temporally, the last ATP-dependent step in exocytosis is closely associated with docking and that docked granules reach fusion competence only after subsequent steps.

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Language(s): eng - English
 Dates: 1995-10-021995-06-221995-10-021995-11-01
 Publication Status: Published in print
 Pages: 12
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 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: Neuron
Source Genre: Journal
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Publ. Info: Cambridge, Mass. : Cell Press
Pages: - Volume / Issue: 15 (5) Sequence Number: - Start / End Page: 1085 - 1096 Identifier: ISSN: 0896-6273
CoNE: https://pure.mpg.de/cone/journals/resource/954925560565