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  A data set from flash X-ray imaging of carboxysomes.

Hantke, M. F., Hasse, D., Ekeberg, T., John, K., Svenda, M., Loh, D., et al. (2016). A data set from flash X-ray imaging of carboxysomes. Scientific Data, 3: 160061. doi:10.1038/sdata.2016.61.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002B-75F9-2 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002B-75FC-B
Genre: Journal Article

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 Creators:
Hantke, M. F., Author
Hasse, D., Author
Ekeberg, T., Author
John, K., Author
Svenda, M., Author
Loh, D., Author
Martin, A. V., Author
Timneanu, N., Author
Larsson, D. S. D., Author
van der Schot, G., Author
Carlsson, G. H., Author
Ingelman, M., Author
Andreasson, J., Author
Westphal, D., Author
Iwan, B., Author
Uetrecht, C., Author
Bielecki, J., Author
Liang, M., Author
Stellato, F., Author
DePonte, D. P., Author
Bari, S.1, Author              Hartmann, R., AuthorKimmel, N., AuthorKirian, R. A., AuthorSeibert, M. M., AuthorMühlig, K., AuthorSchorb, S., AuthorFerguson, K., AuthorBostedt, C., AuthorCarron, S., AuthorBozek, J. D., AuthorRolles, D., AuthorRudenko, A., AuthorFoucar, L., AuthorEpp, S. W., AuthorChapman, H. N., AuthorBarty, A., AuthorAndersson, I., AuthorHajdu, J., AuthorMaia, F. R. N. C., Author more..
Affiliations:
1Research Group of Structural Dynamics of (Bio)Chemical Systems, MPI for Biophysical Chemistry, Max Planck Society, ou_578564              

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 Abstract: Ultra-intense femtosecond X-ray pulses from X-ray lasers permit structural studies on single particles and biomolecules without crystals. We present a large data set on inherently heterogeneous, polyhedral carboxysome particles. Carboxysomes are cell organelles that vary in size and facilitate up to 40% of Earth's carbon fixation by cyanobacteria and certain proteobacteria. Variation in size hinders crystallization. Carboxysomes appear icosahedral in the electron microscope. A protein shell encapsulates a large number of Rubisco molecules in paracrystalline arrays inside the organelle. We used carboxysomes with a mean diameter of 115±26 nm from Halothiobacillus neapolitanus. A new aerosol sample-injector allowed us to record 70,000 low-noise diffraction patterns in 12 min. Every diffraction pattern is a unique structure measurement and high-throughput imaging allows sampling the space of structural variability. The different structures can be separated and phased directly from the diffraction data and open a way for accurate, high-throughput studies on structures and structural heterogeneity in biology and elsewhere.

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Language(s): eng - English
 Dates: 2016-08-01
 Publication Status: Published online
 Pages: -
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 Rev. Method: Peer
 Identifiers: DOI: 10.1038/sdata.2016.61
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Title: Scientific Data
Source Genre: Journal
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Pages: 8 Volume / Issue: 3 Sequence Number: 160061 Start / End Page: - Identifier: -