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  Evaluating atomic models of F-actin with an undecagold-tagged phalloidin derivative

Steinmetz, M. O., Stoffler, D., Müller, S. A., Jahn, W., Wolpensinger, B., Goldie, K. N., et al. (1998). Evaluating atomic models of F-actin with an undecagold-tagged phalloidin derivative. Journal of Molecular Biology (London), 276(1), 1-6. doi:10.1006/jmbi.1997.1529.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002B-78F8-6 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002B-78F9-4
Genre: Journal Article
Alternative Title : Evaluating atomic models of F-actin with an undecagold-tagged phalloidin derivative

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JMolBiol_276_1998_1.pdf (Any fulltext), 501KB
 
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 Creators:
Steinmetz, Michel O., Author
Stoffler, Daniel, Author
Müller, Shirley A., Author
Jahn, Werner1, Author              
Wolpensinger, Bettina, Author
Goldie, Kenneth N., Author
Engel, Andreas, Author
Faulstich, Heinz2, Author              
Aebi, Ueli, Author
Affiliations:
1Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497712              
2Department of Molecular Cell Research, Max Planck Institute for Medical Research, Max Planck Society, ou_1497703              

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Free keywords: F-actin; phalloidin; scanning transmission electron microscopy; image processing; 3-D reconstructions
 Abstract: We have prepared an undecagold-tagged phalloidin derivative to determine this mushroom toxin's binding site and orientation within the F-actin filament by scanning transmission electron microscopy (STEM) and 3-D helical reconstruction. Remarkably, when stoichiometrically bound to F-actin, the undecagold moiety of the derivative could be directly visualized by STEM along the two half-staggered long-pitch helical strands of single filaments. Most importantly, the structural data obtained when combined with various biochemical constraints enabled us to critically evaluate two distinct atomic models of the F-actin filament (i.e. the Holmes-Lorenz versus the Schutt-Lindberg model). Taken together, our data are in excellent agreement with the Holmes-Lorenz model.

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Language(s): eng - English
 Dates: 1997-11-031997-07-291997-11-121998-02-13
 Publication Status: Published in print
 Pages: 6
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: Journal of Molecular Biology (London)
  Other : J Mol Biol
Source Genre: Journal
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Publ. Info: London : Academic Press
Pages: - Volume / Issue: 276 (1) Sequence Number: - Start / End Page: 1 - 6 Identifier: ISSN: 0022-2836
CoNE: https://pure.mpg.de/cone/journals/resource/954922646042