Testing gene function early in the B cell lineage in mb1-cre mice

Hobeika, E.; Thiemann, S.; Storch, B.; Jumaa, H.; Nielsen, P. J.; Pelanda, R.; Reth, M.

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Testing gene function early in the B cell lineage in mb1-cre mice

Hobeika, E., Thiemann, S., Storch, B., Jumaa, H., Nielsen, P. J., Pelanda, R., et al. (2006). Testing gene function early in the B cell lineage in mb1-cre mice. Proceedings of the National Academy of Sciences of the USA, 13789-13794.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-002B-883A-4 Version Permalink: https://hdl.handle.net/21.11116/0000-0001-E5EC-3

Genre: Journal Article

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Creators:
Hobeika, E.¹, Author
Thiemann, S., Author
Storch, B.¹, Author
Jumaa, H.¹, Author
Nielsen, P. J.², Author
Pelanda, R.¹, Author
Reth, M.¹, Author

Affiliations:
1Research Group and Chair of Molecular Immunology of the University of Freiburg, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243645
2Max Planck Society, ou_persistent13

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Free keywords: Dnmt1; SRp20; loxP; enhanced yellow fluorescent protein; lymphocyte

Abstract: The mb1 gene encodes the Ig-α signaling subunit of the B cell antigen receptor and is expressed exclusively in B cells beginning at the very early pro-B cell stage in the bone marrow. We examine here the efficacy of the mb1 gene as a host locus for cre recombinase expression in B cells. We show that by integrating a humanized cre recombinase into the mb1 locus we obtain extraordinarily efficient recombination of loxP sites in the B cell lineage. The results from a variety of reporter genes including the splicing factor SRp20 and the DNA methylase Dnmt1 suggest that mb1-cre is probably the best model so far described for pan-B cell-specific cre expression. The availability of a mouse line with efficient cre-mediated recombination at an early developmental stage in the B lineage provides an opportunity to study the role of various genes specifically in B cell development and function.

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Language(s): eng - English

Dates: Date issued: 2006-09-12

Publication Status: Issued

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Rev. Type: Peer

Identifiers: eDoc: 293405

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Title: Proceedings of the National Academy of Sciences of the USA

Source Genre: Journal

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Pages: - Volume / Issue: - Sequence Number: - Start / End Page: 13789 - 13794 Identifier: -