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  Critical role of lysine 134 methylation on histone H2AX for γ-H2AX production and DNA repair

Sone, K., Piao, L., Nakakido, M., Ueda, K., Jenuwein, T., Nakamura, Y., et al. (2014). Critical role of lysine 134 methylation on histone H2AX for γ-H2AX production and DNA repair. Nature Communications, 5: 5691 (2014), pp. 5691. doi:10.1038/ncomms6691.

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Sone, Kenbun1, Author
Piao, Lianhua1, Author
Nakakido, Makoto1, Author
Ueda, Koji1, Author
Jenuwein, Thomas2, Author              
Nakamura, Yusuke1, Author
Hamamoto, Ryuji1, Author
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1External Organizations, ou_persistent22              
2Department of Epigenetics, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243644              

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 Abstract: The presence of phosphorylated histone H2AX (γ-H2AX) is associated with the local activation of DNA-damage repair pathways. Although γ-H2AX deregulation in cancer has previously been reported, the molecular mechanism involved and its relationship with other histone modifications remain largely unknown. Here we find that the histone methyltransferase SUV39H2 methylates histone H2AX on lysine 134. When H2AX was mutated to abolish K134 methylation, the level of γ-H2AX became significantly reduced. We also found lower γ-H2AX activity following the introduction of double-strand breaks in Suv39h2 knockout cells or on SUV39H2 knockdown. Tissue microarray analyses of clinical lung and bladder tissues also revealed a positive correlation between H2AX K134 methylation and γ-H2AX levels. Furthermore, introduction of K134-substituted histone H2AX enhanced radio- and chemosensitivity of cancer cells. Overall, our results suggest that H2AX methylation plays a role in the regulation of γ-H2AX abundance in cancer.

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Language(s): eng - English
 Dates: 2014-12-09
 Publication Status: Published online
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 Rev. Type: Peer
 Identifiers: DOI: 10.1038/ncomms6691
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Title: Nature Communications
  Abbreviation : Nat. Commun.
Source Genre: Journal
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Publ. Info: London : Nature Publishing Group
Pages: - Volume / Issue: 5 Sequence Number: 5691 (2014) Start / End Page: 5691 Identifier: ISSN: 2041-1723
CoNE: https://pure.mpg.de/cone/journals/resource/2041-1723