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  Nontransformed, GM-CSF-dependent macrophage lines are a unique model to study tissue macrophage functions

Fejer, G., Wegner, M. D., Györy, I., Cohen, I., Engelhard, P., Voronov, E., et al. (2013). Nontransformed, GM-CSF-dependent macrophage lines are a unique model to study tissue macrophage functions. Proceedings of the National Academy of Sciences Uzs.A., 110, E-2191-E-2198.

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 Creators:
Fejer, György1, Author           
Wegner, Mareike Dorothee2, Author
Györy, Ildiko3, Author           
Cohen, Idan4, Author           
Engelhard, Peggy, Author
Voronov, Elena, Author
Manke, Thomas5, Author           
Ruzsics, Zsolt, Author
Dölken, Lars, Author
Prazeres da Costa, Olivia, Author
Branzk, Nora1, Author           
Huber, Michael6, Author           
Prasse, Antje, Author
Schneider, Robert4, Author           
Apte, Ron N., Author
Galanos, Chris7, Author           
Freudenberg, Marina A.1, Author           
Affiliations:
1Department of Developmental Immunology, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243647              
2Max Planck Society, ou_persistent13              
3Department of Cellular and Molecular Immunology, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243641              
4Spemann Laboratory, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243655              
5Department of Epigenetics, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243644              
6Research Group and Chair of Molecular Immunology of the University of Freiburg, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243645              
7Emeritus Group: Cellular Immunology, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243649              

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Free keywords: LPS recognition; innate immunity
 Abstract: Macrophages are diverse cell types in the first line of antimicrobial defense. Only a limited number of primary mouse models exist to study their function. Bone marrow-derived, macrophage-CSF-induced cells with a limited life span are the most common source. We report here a simple method yielding self-renewing, nontransformed, GM-CSF/signal transducer and activator of transcription 5-dependent macrophages (Max Planck Institute cells) from mouse fetal liver, which reflect the innate immune characteristics of alveolar macrophages. Max Planck Institute cells are exquisitely sensitive to selected microbial agents, including bacterial LPS, lipopeptide, Mycobacterium tuberculosis, cord factor, and adenovirus and mount highly proinflammatory but no anti-inflammatory IL-10 responses. They show a unique pattern of innate responses not yet observed in other mononuclear phagocytes. This includes differential LPS sensing and an unprecedented regulation of IL-1α production upon LPS exposure, which likely plays a key role in lung inflammation in vivo. In conclusion, Max Planck Institute cells offer an useful tool to study macrophage biology and for biomedical science.

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Language(s): eng - English
 Dates: 2013
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 674924
 Degree: -

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Title: Proceedings of the National Academy of Sciences Uzs.A.
  Alternative Title : PNAS
Source Genre: Journal
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Pages: - Volume / Issue: 110 Sequence Number: - Start / End Page: E-2191 - E-2198 Identifier: -