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  Satb1 and Satb2 Are Dispensable for X Chromosome Inactivation in Mice

Nechanitzky, R., Dávila, A., Savarese, F., Fietze, S., & Grosschedl, R. (2012). Satb1 and Satb2 Are Dispensable for X Chromosome Inactivation in Mice. Developmental Cell, 23, 866-871. doi:10.1016/j.devcel.2012.09.018.

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Nechanitzky 2012_Dev Cell.pdf (Publisher version), 339KB
 
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Nechanitzky, Robert1, Author              
Dávila, Amparo1, Author              
Savarese, Fabio1, Author              
Fietze, Stefanie1, Author
Grosschedl, Rudolf1, Author              
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1Department of Cellular and Molecular Immunology, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243641              

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 Abstract: Satb1 and Satb2 have been recently described as regulators of embryonic stem (ES) cell pluripotency and as silencing factors in X chromosome inactivation. The influence of the pluripotency machinery on X chromosome inactivation and the lack of an X chromosome inactivation defect in Satb1-/- and Satb2-/- mice raise the question of whether or not Satb proteins are directly and/or redundantly involved in this process. Here, we analyzed X chromosome inactivation in fibroblastic cells that were derived from female Satb1-/-Satb2-/- embryos. By fluorescence in situ hybridization to visualize Xist RNA and by immunohistochemistry to detect H3K27me3 histone modifications, we found that female Satb1-/-Satb2-/- fibroblastic cells contain proper Barr bodies. Moreover, we did not detect an upregulation of X-linked genes, suggesting that Satb proteins are dispensable for X chromosome inactivation in mice.

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Language(s): eng - English
 Dates: 2012-10-16
 Publication Status: Published online
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 Rev. Type: Peer
 Identifiers: DOI: 10.1016/j.devcel.2012.09.018
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Title: Developmental Cell
Source Genre: Journal
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Publ. Info: Cambridge, Mass. : Cell Press
Pages: - Volume / Issue: 23 Sequence Number: - Start / End Page: 866 - 871 Identifier: ISSN: 1534-5807
CoNE: https://pure.mpg.de/cone/journals/resource/111006902714134