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  A transcription factor-based mechanism for mouse heterochromatin formation

Bulut-Karslioglu, A., Perrera, V., Scaranaro, M., de la Rosa-Velazquez, I. A., van de Nobelen, S., Shukeir, N., et al. (2012). A transcription factor-based mechanism for mouse heterochromatin formation. Nature Structural and Molecular Biology, 19, 1023-1030. doi:10.1038/nsmb.2382.

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Bulut-Karslioglu, Aydan1, Author
Perrera, Valentina1, Author              
Scaranaro, Manuela2, Author
de la Rosa-Velazquez, Inti Alberto1, Author              
van de Nobelen, Suzanne1, Author
Shukeir, Nicholas1, Author              
Popow, Johannes2, Author
Gerle, Borbala2, Author
Opravil, Susanne2, Author
Pagani, Michaela2, Author
Meidhof, Simone2, Author
Brabletz, Thomas2, Author
Manke, Thomas1, Author              
Lachner, Monika1, Author
Jenuwein, Thomas1, Author              
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1Department of Epigenetics, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243644              
2External Organizations, ou_persistent22              

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 Abstract: Heterochromatin is important for genome integrity and stabilization of gene-expression programs. We have identified the transcription factors Pax3 and Pax9 as redundant regulators of mouse heterochromatin, as they repress RNA output from major satellite repeats by associating with DNA within pericentric heterochromatin. Simultaneous depletion of Pax3 and Pax9 resulted in dramatic derepression of major satellite transcripts, persistent impairment of heterochromatic marks and defects in chromosome segregation. Genome-wide analyses of methylated histone H3 at Lys9 showed enrichment at intergenic major satellite repeats only when these sequences retained intact binding sites for Pax and other transcription factors. Additionally, bioinformatic interrogation of all histone methyltransferase Suv39h-dependent heterochromatic repeat regions in the mouse genome revealed a high concordance with the presence of transcription factor binding sites. These data define a general model in which reiterated arrangement of transcription factor binding sites within repeat sequences is an intrinsic mechanism of the formation of heterochromatin.

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Language(s): eng - English
 Dates: 2012-09-162012-10
 Publication Status: Published in print
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 Rev. Type: Peer
 Identifiers: DOI: 10.1038/nsmb.2382
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Title: Nature Structural and Molecular Biology
  Other : Nature Struct Biol
Source Genre: Journal
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Publ. Info: New York, NY : Nature Pub. Group
Pages: - Volume / Issue: 19 Sequence Number: - Start / End Page: 1023 - 1030 Identifier: ISSN: 1545-9993
CoNE: https://pure.mpg.de/cone/journals/resource/954925603763