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  A transcription factor-based mechanism for mouse heterochromatin formation

Bulut-Karslioglu, A., Perrera, V., Scaranaro, M., de la Rosa-Velazquez, I. A., van de Nobelin, S., Shukeir, N., et al. (2012). A transcription factor-based mechanism for mouse heterochromatin formation. Nature Structural & Molecular Biology, 19, 1023-1030.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002B-8CC4-C Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002B-8CC8-4
Genre: Journal Article

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 Creators:
Bulut-Karslioglu, Aydan1, Author
Perrera, Valentina2, Author              
Scaranaro, Manuela, Author
de la Rosa-Velazquez, Inti Alberto2, Author              
van de Nobelin, Suzanne1, Author
Shukeir, Nicholas1, Author
Popow, Johannes, Author
Gerle, Borbala, Author
Opravil, Susanne, Author
Pagani, Michaela, Author
Meidhof, Simone, Author
Brabletz, Thomas, Author
Manke, Thomas2, Author              
Lachner, Monika1, Author
Jenuwein, Thomas2, Author              
Affiliations:
1Max Planck Society, ou_persistent13              
2Department of Epigenetics, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243644              

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 Abstract: Heterochromatin is important for genome integrity and stabilization of gene-expression programs. We have identified the transcription factors Pax3 and Pax9 as redundant regulators of mouse heterochromatin, as they repress RNA output from major satellite repeats by associating with DNA within pericentric heterochromatin. Simultaneous depletion of Pax3 and Pax9 resulted in dramatic derepression of major satellite transcripts, persistent impairment of heterochromatic marks and defects in chromosome segregation. Genome-wide analyses of methylated histone H3 at Lys9 showed enrichment at intergenic major satellite repeats only when these sequences retained intact binding sites for Pax and other transcription factors. Additionally, bioinformatic interrogation of all histone methyltransferase Suv39h-dependent heterochromatic repeat regions in the mouse genome revealed a high concordance with the presence of transcription factor binding sites. These data define a general model in which reiterated arrangement of transcription factor binding sites within repeat sequences is an intrinsic mechanism of the formation of heterochromatin.

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Language(s): eng - English
 Dates: 2012-10
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 634065
 Degree: -

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Title: Nature Structural & Molecular Biology
Source Genre: Journal
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Pages: - Volume / Issue: 19 Sequence Number: - Start / End Page: 1023 - 1030 Identifier: -