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  Drosophila CENH3 Is Sufficient for Centromere Formation

Mendiburo, M. J., Padeken, J., Fülöp, S., Schepers, A., & Heun, P. (2011). Drosophila CENH3 Is Sufficient for Centromere Formation. Science, 334, 686-690.

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Mendiburo, Maria José1, Autor           
Padeken, Jan1, Autor           
Fülöp, Stefanie, Autor
Schepers, Aloys, Autor
Heun, Patrick1, Autor           
Affiliations:
1Department of Cellular and Molecular Immunology, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243641              

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 Zusammenfassung: CENH3 is a centromere-specific histone H3 variant essential for kinetochore assembly. Despite its central role in centromere function, there has been no conclusive evidence supporting CENH3 as sufficient to determine centromere identity. To address this question, we artificially targeted Drosophila CENH3 (CENP-A/CID) as a CID-GFP-LacI fusion protein to stably integrated lac operator (lacO) arrays. This ectopic CID focus assembles a functional kinetochore and directs incorporation of CID molecules without the LacI-anchor, providing evidence for the self-propagation of the epigenetic mark. CID-GFP-LacI-bound extrachromosomal lacO plasmids can assemble kinetochore proteins and bind microtubules, resulting in their stable transmission for several cell generations even after eliminating CID-GFP-LacI. We conclude that CID is both necessary and sufficient to serve as an epigenetic centromere mark and nucleate heritable centromere function.

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Sprache(n): eng - English
 Datum: 2011-11-04
 Publikationsstatus: Erschienen
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 Ort, Verlag, Ausgabe: -
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 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: eDoc: 577782
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Titel: Science
Genre der Quelle: Zeitschrift
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Ort, Verlag, Ausgabe: -
Seiten: - Band / Heft: 334 Artikelnummer: - Start- / Endseite: 686 - 690 Identifikator: -