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  Biological and mathematical modeling of melanocyte development

Luciani, F., Champeval, D., Herbette, A., Denat, L., Aylaj, B., Martinozzi, S., et al. (2011). Biological and mathematical modeling of melanocyte development. Development, 138, 3943-3954.

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 Creators:
Luciani, Flavie, Author
Champeval, Delphine, Author
Herbette, Aurélle, Author
Denat, Laurence, Author
Aylaj, Bouchra, Author
Martinozzi, Silvia, Author
Ballotti, Robert, Author
Kemler, Rolf1, Author           
Goding, Colin R., Author
De Vuyst, Florian, Author
Larue, Lionel, Author
Delmas, Véronique, Author
Affiliations:
1Emeritus Group: Molecular Embryology, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243656              

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Free keywords: Mitf; Dct; β-Catenin; Melanoblast; Mouse
 Abstract: We aim to evaluate environmental and genetic effects on the expansion/proliferation of committed single cells during embryonic development, using melanoblasts as a paradigm to model this phenomenon. Melanoblasts are a specific type of cell that display extensive cellular proliferation during development. However, the events controlling melanoblast expansion are still poorly understood due to insufficient knowledge concerning their number and distribution in the various skin compartments. We show that melanoblast expansion is tightly controlled both spatially and temporally, with little variation between embryos. We established a mathematical model reflecting the main cellular mechanisms involved in melanoblast expansion, including proliferation and migration from the dermis to epidermis. In association with biological information, the model allows the calculation of doubling times for melanoblasts, revealing that dermal and epidermal melanoblasts have short but different doubling times. Moreover, the number of trunk founder melanoblasts at E8.5 was estimated to be 16, a population impossible to count by classical biological approaches. We also assessed the importance of the genetic background by studying gain- and loss-of-function β-catenin mutants in the melanocyte lineage. We found that any alteration of β-catenin activity, whether positive or negative, reduced both dermal and epidermal melanoblast proliferation. Finally, we determined that the pool of dermal melanoblasts remains constant in wild-type and mutant embryos during development, implying that specific control mechanisms associated with cell division ensure half of the cells at each cell division to migrate from the dermis to the epidermis. Modeling melanoblast expansion revealed novel links between cell division, cell localization within the embryo and appropriate feedback control through β-catenin.

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Language(s): eng - English
 Dates: 2011
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 577569
 Degree: -

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Title: Development
Source Genre: Journal
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Pages: - Volume / Issue: 138 Sequence Number: - Start / End Page: 3943 - 3954 Identifier: -