English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Heterochromatin boundaries are hotspots for de novo kinetochore formation

Olszak, A. M., van Essen, D., Pereira, A., Diehl, S., Manke, T., Maiato, H., et al. (2011). Heterochromatin boundaries are hotspots for de novo kinetochore formation. Nature Cell Biology, 13, 799-808.

Item is

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Olszak, Agata M., Author
van Essen, Dominic1, Author              
Pereira, António, Author
Diehl, Sarah, Author
Manke, Thomas2, Author              
Maiato, Helder, Author
Saccani, Simona1, Author              
Heun, Patrick1, Author              
Affiliations:
1Department of Cellular and Molecular Immunology, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243641              
2Department of Epigenetics, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243644              

Content

show
hide
Free keywords: -
 Abstract: The centromere-specific histone H3 variant CENH3 (also known as CENP-A) is considered to be an epigenetic mark for establishment and propagation of centromere identity. Pulse induction of CENH3 (Drosophila CID) in Schneider S2 cells leads to its incorporation into non-centromeric regions and generates CID islands that resist clearing from chromosome arms for multiple cell generations. We demonstrate that CID islands represent functional ectopic kinetochores, which are non-randomly distributed on the chromosome and show a preferential localization near telomeres and pericentric heterochromatin in transcriptionally silent, intergenic chromatin domains. Although overexpression of heterochromatin protein 1 (HP1) or increasing histone acetylation interferes with CID island formation on a global scale, induction of a locally defined region of synthetic heterochromatin by targeting HP1-LacI fusions to stably integrated Lac operator arrays produces a proximal hotspot for CID deposition. These data indicate that the characteristics of regions bordering heterochromatin promote de novo kinetochore assembly and thereby contribute to centromere identity.

Details

show
hide
Language(s): eng - English
 Dates: 2011
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 577823
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Nature Cell Biology
  Alternative Title : Nat. Cell Biol.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 13 Sequence Number: - Start / End Page: 799 - 808 Identifier: -