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  Methylation of H2AR29 is a novel repressive PRMT6 target

Waldmann, T., Izzo, A., Kamieniarz, K., Richter, F., Vogler, C., Sarg, B., et al. (2011). Methylation of H2AR29 is a novel repressive PRMT6 target. Epigenetics & Chromatin, 4, 1-10.

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 Creators:
Waldmann, Tanja1, Author              
Izzo, Annalisa1, Author              
Kamieniarz, Kinga1, Author              
Richter, Florian2, Author
Vogler, Christine1, Author              
Sarg, Bettina, Author
Lindner, Herbert, Author
Young, Nicolas L., Author
Mittler, Gerhard3, Author              
Garcia, Benjamin A., Author
Schneider, Robert1, Author              
Affiliations:
1Spemann Laboratory, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243655              
2Max Planck Society, ou_persistent13              
3Department of Cellular and Molecular Immunology, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243641              

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 Abstract: BACKGROUND: Covalent histone modifications are central to all DNA-dependent processes. Modifications of histones H3 and H4 are becoming well characterised, but knowledge of how H2A modifications regulate chromatin dynamics and gene expression is still very limited. RESULTS: To understand the function of H2A modifications, we performed a systematic analysis of the histone H2A methylation status. We identified and functionally characterised two new methylation sites in H2A: R11 (H2AR11) and R29 (H2AR29). Using an unbiased biochemical approach in combination with candidate assays we showed that protein arginine methyltransferase (PRMT) 1 and PRMT6 are unique in their ability to catalyse these modifications. Importantly we found that H2AR29me2 is specifically enriched at genes repressed by PRMT6, implicating H2AR29me2 in transcriptional repression. CONCLUSIONS: Our data establishes R11 and R29 as new arginine methylation sites in H2A. We identified the specific modifying enzymes involved, and uncovered a novel functional role of H2AR29me2 in gene silencing in vivo. Thus this work reveals novel insights into the function of H2A methylation and in the mechanisms of PRMT6-mediated transcriptional repression.

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Language(s): eng - English
 Dates: 2011
 Publication Status: Published in print
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 Rev. Type: Peer
 Identifiers: eDoc: 578724
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Title: Epigenetics & Chromatin
Source Genre: Journal
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Pages: - Volume / Issue: 4 Sequence Number: - Start / End Page: 1 - 10 Identifier: -