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  Cdx1::Cre Allele for Gene Analysis in the Extraembryonic Ectoderm and the Three Germ Layers of Mice at Mid-Gastrulation

Hierholzer, A., & Kemler, R. (2009). Cdx1:Cre Allele for Gene Analysis in the Extraembryonic Ectoderm and the Three Germ Layers of Mice at Mid-Gastrulation. Genesis, 47, 204-209.

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資料種別: 学術論文

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 作成者:
Hierholzer, Andreas1, 著者           
Kemler, Rolf1, 著者           
所属:
1Emeritus Group: Molecular Embryology, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243656              

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キーワード: Cre recombinase; Cdx1 expression; trophectoderm; primitive streak; placental development
 要旨: Transgenic mice with a defined cell- or tissues-specific expression of Cre-recombinase are essential tools to study gene function. Here we report the generation and analysis of a transgenic mouse line (Cdx1::Cre) with restricted Cre-expression from Cdx1 regulatory elements. The expression of Cre-recombinase mimicked the endogenous expression pattern of Cdx1 at midgastrulation (from E7.5 to early headfold stage) inducing recombination in the three germlayers of the primitive streak region throughout the posterior embryo and caudal to the heart. This enables gene modifications to investigate patterning of the caudal embryo during and after gastrulation. Interestingly, we identified Cdx1 expression in the trophectoderm (TE) of blastocyst stage embryos. Concordantly, we detected extensive Cre-mediated recombination in the polar TE and, although to lesser extent, in the mural TE. In E7.5 postimplantation embryos, almost all cells of the extraembryonic ectoderm (ExE), which are derived from the polar TE, are recombined although the ExE itself is negative for Cdx1 and Cre at this stage. These results indicate that Cdx1::Cre mice are also a valuable tool to study gene function in tissues essential for placental development.

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言語: eng - English
 日付: 2009
 出版の状態: 出版
 ページ: -
 出版情報: -
 目次: -
 査読: 査読あり
 識別子(DOI, ISBNなど): eDoc: 456784
 学位: -

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出版物 1

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出版物名: Genesis
種別: 学術雑誌
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出版社, 出版地: -
ページ: - 巻号: 47 通巻号: - 開始・終了ページ: 204 - 209 識別子(ISBN, ISSN, DOIなど): -