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  Detection of protein complex interactions via a Blue Native-PAGE retardation assay

Swamy, M., Molnar, E., Bock, T., Bausch-Fluck, D., Wollscheid, B., & Schamel, W. W. A. (2009). Detection of protein complex interactions via a Blue Native-PAGE retardation assay. Analytical Biochemstry, 392, 177-179.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-002B-8FA8-7 Version Permalink: https://hdl.handle.net/11858/00-001M-0000-002B-8FA9-5
Genre: Journal Article

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 Creators:
Swamy, Mahima1, Author           
Molnar, Eszter2, Author
Bock, Thomas1, Author           
Bausch-Fluck, Damaris, Author
Wollscheid, Bernd1, Author           
Schamel, Wolfgang W. A.1, Author           
Affiliations:
1Research Group and Chair of Molecular Immunology of the University of Freiburg, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243645              
2Max Planck Society, ou_persistent13              

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 Abstract: We describe the Blue Native (BN)-PAGE retardation assay for the detection of interactions of biomolecules with protein complexes. Potential interactors of proteins are included in the BN gel matrix, resulting in retardation of proteins that interact with the added molecule. After validation using the T-cell antigen receptor, we applied the assay for a general identification of dextran interactors in combination with mass spectroscopy. The proteomic screen revealed triosephosphate isomerase oligomer as a dextranbinding, high MR complex.

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Language(s): eng - English
 Dates: 2009
 Publication Status: Issued
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 464645
 Degree: -

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Title: Analytical Biochemstry
Source Genre: Journal
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Pages: - Volume / Issue: 392 Sequence Number: - Start / End Page: 177 - 179 Identifier: -