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  Two-Dimensional Blue Native Polyacrylamide Gel Electrophoresis

Schamel, W. W. A. (2008). Two-Dimensional Blue Native Polyacrylamide Gel Electrophoresis. Current Protocols in Cell Biology, 38, 6.10.1-6.10.21.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-002B-900D-D Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002B-900E-B
Genre: Journal Article

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 Creators:
Schamel, Wolfgang W. A.1, Author              
Affiliations:
1Research Group and Chair of Molecular Immunology of the University of Freiburg, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243645              

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Free keywords: multiprotein complex; native; gel electrophoresis; two-dimensional; Coomassie blue; protein-protein interaction
 Abstract: Multiprotein complexes play crucial roles in nearly all cell biological processes. Blue Native Polyacrylamide Gel Electrophoresis (BN-PAGE) is a powerful method to study these complexes. It is a native protein separation method that relies on the dye Coomassie blue to confer negative charge for separation. It has a higher resolution than gel filtration or sucrose density ultracentrifugation and can be used for protein complexes from 10 kDa to 10 MDa. If a second-dimension SDS-PAGE is applied (two-dimensional BN/SDS-PAGE), the size, subunit composition, and relative abundance of the different multiprotein complexes can be studied. In recent years, there has been a large increase in the number of publications where BN-PAGE was used to study protein-protein interactions. Here, we give detailed protocols for the separation of multiprotein complexes by two-dimensional BN/SDS-PAGE and for a related technique to determine the stoichiometry of these complexes.

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Language(s): eng - English
 Dates: 2008-03
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 402241
 Degree: -

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Title: Current Protocols in Cell Biology
Source Genre: Journal
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Publ. Info: -
Pages: - Volume / Issue: 38 Sequence Number: - Start / End Page: 6.10.1 - 6.10.21 Identifier: -