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  The multidomain protein Brpf1 binds histones and is required for Hox gene expression and segmental identity

Laue, K., Daujat, S., Crump Gage, J., Plaster, N., Roehl, H. H., van Bebber, F., et al. (2008). The multidomain protein Brpf1 binds histones and is required for Hox gene expression and segmental identity. Development, 135, 1935-1946.

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 Creators:
Laue, Kathrin1, Author              
Daujat, Sylvain2, Author              
Crump Gage, Justin, Author
Plaster, Nikki, Author
Roehl, Henry H., Author
van Bebber, F., Author
Busch-Nentwich, E., Author
Dahm, R., Author
Frohnhöfer, H. G., Author
Geiger, H., Author
Gilmour, S., Author
Holley, S., Author
Hooge, J., Author
Jülich, J., Author
Knaut, H., Author
Maderspacher, F., Author
Maischein, H.-M., Author
Neumann, C., Author
Nicolson, T., Author
Nüsslein-Volhard, C., Author
Schoenberger, U., AuthorSeiler, C., AuthorSöllner, C., AuthorSonawane, M., AuthorWehner, A., AuthorErker, P., AuthorHabeck, H., AuthorHagner, U., AuthorHennen, C., AuthorKaps, E., AuthorKirchner, A., AuthorKoblitzek, T., AuthorLangenreich, U., AuthorLoesche, C., AuthorMetzger, C., AuthorNordin, R., AuthorOdenthal, J., AuthorPezzuti, M., AuthorSchlombs, K., AuthordeSatana-Stamm, J., AuthorTrowe, T., AuthorVacun, G., AuthorWalderich, B.3, Author              Walker, A., AuthorWeiler, C., AuthorKimmel, Charles B., AuthorSchneider, Robert2, Author              Hammerschmidt, Matthias1, Author               more..
Affiliations:
1Georges Köhler Laboratory, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243653              
2Spemann Laboratory, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243655              
3Department of Developmental Immunology, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243647              

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Free keywords: Brpf1; Bromodomain; PWWP domain; Moz; Hox gene expression; Craniofacial development; Cranial neural crest; Pharyngeal arch; Anterior-posterior patterning; Homeotic transformation; Zebrafish
 Abstract: The Trithorax group (TrxG) is composed of diverse, evolutionary conserved proteins that form chromatin-associated complexes accounting for epigenetic transcriptional memory. However, the molecular mechanisms by which particular loci are marked for reactivation after mitosis are only partially understood. Here, based on genetic analyses in zebrafish, we identify the multidomain protein Brpf1 as a novel TrxG member with a central role during development. brpf1 mutants display anterior transformations of pharyngeal arches due to progressive loss of anterior Hox gene expression. Brpf1 functions in association with the histone acetyltransferase Moz (Myst3), an interaction mediated by the N-terminal domain of Brpf1, and promotes histone acetylation in vivo. Brpf1 recruits Moz to distinct sites of active chromatin and remains at chromosomes during mitosis, mediated by direct histone binding of its bromodomain, which has a preference for acetylated histones, and its PWWP domain, which binds histones independently of their acetylation status. This is the first demonstration of histone binding for PWWP domains. Mutant analyses further show that the PWWP domain is absolutely essential for Brpf1 function in vivo. We conclude that Brpf1, coordinated by its particular set of domains, acts by multiple mechanisms to mediate Moz-dependent histone acetylation and to mark Hox genes for maintained expression throughout vertebrate development.

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Language(s): eng - English
 Dates: 2008
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 400766
 Degree: -

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Title: Development
Source Genre: Journal
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Pages: - Volume / Issue: 135 Sequence Number: - Start / End Page: 1935 - 1946 Identifier: -