English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
 
 
DownloadE-Mail
  Wnt signaling interacts with Shh to regulate taste papilla development

Iwatsuki, K., Liu, H.-X., Gründer, A., Singer, M., Lane, T. F., Grosschedl, R., et al. (2007). Wnt signaling interacts with Shh to regulate taste papilla development. Proceedings of the National Academy of Sciences of the United States of America, 104, 2253-2258. doi:10.1073/pnas.0607399104.

Item is

Files

show Files

Locators

show
hide
Locator:
https://www.pnas.org/content/104/7/2253 (Publisher version)
Description:
-
OA-Status:

Creators

show
hide
 Creators:
Iwatsuki, Ken1, Author
Liu, Hong-Xiang1, Author
Gründer, Albert2, Author
Singer, Meredith1, Author
Lane, Timothy F.1, Author
Grosschedl, Rudolf2, Author           
Mistretta, Charlotte1, Author
Margolskee, Robert F.1, Author
Affiliations:
1External Organizations, ou_persistent22              
2Department of Cellular and Molecular Immunology, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243641              

Content

show
hide
Free keywords: β-catenin; Lef1; Wnt10b; fungiform papilla
 Abstract: Wnt and Shh signaling pathways are critical for the development and maturation of many epithelial tissues. Both pathways have roles in stem cell maintenance, tissue development, and tumorigenesis. However, linkage between these pathways in mammalian systems had not been well established. Here, we report that Shh expression in fungiform papillae and formation of normal mature fungiform papillae depend on signaling through Wnt and β-catenin. We observed that during fungiform papilla formation in mice, Shh and components of the Wnt/β-catenin signaling pathway are expressed together in the developing placode. The elimination of Wnt/β-catenin signaling in either Lef1 or Wnt10b knockout mice resulted in down-regulation of Shh expression. In addition, the size and number of fungiform papillae were greatly reduced in Lef1 knockout mice. By examining embryonic mouse tongues in culture we determined that activation of Wnt/β-catenin signaling up-regulates Shh expression. We observed that blocking Shh signaling in cultured tongue explants enhanced papillae formation and was accompanied by an up-regulation of Wnt/β-catenin signaling, indicating that Shh inhibits the Wnt/β-catenin pathway. Exogenously added Shh suppressed expression of endogenous Shh and inhibited Wnt/β-catenin signaling (assessed in TOPGAL mice), further implicating Shh as an inhibitor of the Wnt/β-catenin pathway. Our observations indicate that Wnt/β-catenin signaling and interactions between the Wnt and Shh pathways play essential roles in the development of fungiform papillae.

Details

show
hide
Language(s): eng - English
 Dates: 2007-02-13
 Publication Status: Published online
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1073/pnas.0607399104
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Proceedings of the National Academy of Sciences of the United States of America
  Other : PNAS
  Other : Proceedings of the National Academy of Sciences of the USA
  Abbreviation : Proc. Natl. Acad. Sci. U. S. A.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Washington, D.C. : National Academy of Sciences
Pages: - Volume / Issue: 104 Sequence Number: - Start / End Page: 2253 - 2258 Identifier: ISSN: 0027-8424
CoNE: https://pure.mpg.de/cone/journals/resource/954925427230