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  Granzyme B is expressed in mouse mast cells in vivo and in vitro and causes delayed cell death independent of perforin

Pardo, J., Wallich, R., Ebnet, K., Iden, S., Zentgraf, H., Martin, P., et al. (2007). Granzyme B is expressed in mouse mast cells in vivo and in vitro and causes delayed cell death independent of perforin. Cell Death and Differentiation, 14, 1768-1779.

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 Creators:
Pardo, J.1, Author              
Wallich, R., Author
Ebnet, K., Author
Iden, S., Author
Zentgraf, H.1, Author              
Martin, P.2, Author              
Ekiciler, Aq, Author
Prins, A., Author
Müllbacher, A.3, Author
Huber, M.4, Author              
Simon, M. M.3, Author
Affiliations:
1Metchnikoff Laboratory, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243654              
2Emeritus Group: Cellular Immunology, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243649              
3Max Planck Society, ou_persistent13              
4Research Group and Chair of Molecular Immunology of the University of Freiburg, Max Planck Institute of Immunobiology and Epigenetics, Max Planck Society, ou_2243645              

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Free keywords: mast cells; anoikis; granzmye B
 Abstract: Mast cells respond to pathogens and allergens by secreting a vast array of preformed and newly synthesized mediators, including enzymes, vasoactive amines, lipid mediators, cytokines and chemokines, thereby affecting innate and adaptive immune responses and pathogenesis. Here, we present evidence that skin-, but not lung-associated primary mast cells as well as in vitro-differentiated bone marrow-derived mast cells (BMMC) express granzyme (gzm) B, but not gzmA or perforin (perf). GzmB is associated with cytoplasmic granules of BMMC and secreted after Fcε-receptor-mediated activation. BMMC from wild type but not gzmB-deficient mice cause cell death in susceptible adherent target cells, indicating that the perf-independent cytotoxicity of BMMC is executed by gzmB. Furthermore, gzmB induces a disorganization of endothelial cell-cell contacts. The data suggest that activated mast cells contribute, via secreted gzmB, to cell death, increased vascular permeability, leukocyte extravasation and subsequent inflammatory processes in affected tissues.

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Language(s): eng - English
 Dates: 2007
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 330366
 Degree: -

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Title: Cell Death and Differentiation
Source Genre: Journal
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Pages: - Volume / Issue: 14 Sequence Number: - Start / End Page: 1768 - 1779 Identifier: -